PUBLICATION
Endothermy in the smalleye opah (Lampris incognitus): A potential role for the uncoupling protein sarcolipin
- Authors
- Franck, J.P.C., Slight-Simcoe, E., Wegner, N.C.
- ID
- ZDB-PUB-190405-22
- Date
- 2019
- Source
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 233: 48-52 (Journal)
- Registered Authors
- Franck, Jens
- Keywords
- Calcium ATPase, Endothermy, Gene expression, Lampris guttatus Lampris incognitus, Non-shivering thermogenesis, Opah, Sarcolipin, Sarcoplasmic reticulum
- MeSH Terms
-
- Animals
- Body Temperature Regulation/genetics*
- Calcium-Transporting ATPases/biosynthesis
- Calcium-Transporting ATPases/genetics*
- Cold Temperature
- Fishes/genetics*
- Fishes/physiology
- Muscle Proteins/biosynthesis
- Muscle Proteins/genetics*
- Proteolipids/biosynthesis
- Proteolipids/genetics*
- Sarcoplasmic Reticulum
- Zebrafish/genetics
- PubMed
- 30946978 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Citation
Franck, J.P.C., Slight-Simcoe, E., Wegner, N.C. (2019) Endothermy in the smalleye opah (Lampris incognitus): A potential role for the uncoupling protein sarcolipin. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 233:48-52.
Abstract
Sarcolipin (SLN) is a small transmembrane protein that in mice has been shown to uncouple the calcium ATPase pump of the sarcoplasmic reticulum, resulting in heat production. Mice up-regulate expression of SLN in response to cold challenge. This thermoregulatory mechanism is characterized as non-shivering muscle-based thermogenesis (NST). The current study was conducted to determine if the endothermic fish species, the smalleye opah (Lampris incognitus), has higher levels of sln transcription in tissues thought to be the main source of endothermic heat, namely the red aerobic pectoral fin musculature, which powers continuous swimming in this species. A search of the draft assembly of the opah genome reveals a single sln gene that is 95% identical to the zebrafish sln ortholog at the amino acid level. Quantitative PCR (qPCR) using opah-specific sln shows significantly higher sln transcript levels in the dark red pectoral fin muscle compared to both the light red pectoral muscle and white axial muscle tissues. The high ratio of sln transcripts to CaATPase (serca1) transcripts suggests that opah may utilize a futile calcium cycling NST mechanism in the dark red pectoral fin muscle to generate heat.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping