PUBLICATION
Identification of Potential LncRNA Biomarker of Mercury Compounds in Zebrafish Embryos
- Authors
- Cao, M., Song, F., Yang, X., Peng, L., Cheng, Y., Zheng, Q., Liang, Y., Wang, C.
- ID
- ZDB-PUB-190327-7
- Date
- 2019
- Source
- Chemical Research in Toxicology 32(5): 878-886 (Journal)
- Registered Authors
- Cao, Mengxi, Liang, Yong
- Keywords
- none
- MeSH Terms
-
- Animals
- Biomarkers/metabolism*
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- In Situ Hybridization, Fluorescence
- Locomotion/drug effects
- Mercury Compounds/toxicity*
- RNA, Long Noncoding/genetics
- RNA, Long Noncoding/metabolism*
- Real-Time Polymerase Chain Reaction
- Zebrafish/genetics*
- Zebrafish/growth & development
- PubMed
- 30912647 Full text @ Chem. Res. Toxicol.
- CTD
- 30912647
Citation
Cao, M., Song, F., Yang, X., Peng, L., Cheng, Y., Zheng, Q., Liang, Y., Wang, C. (2019) Identification of Potential LncRNA Biomarker of Mercury Compounds in Zebrafish Embryos. Chemical Research in Toxicology. 32(5):878-886.
Abstract
Heavy metal pollution elicits severe environmental concern and health problem worldwide. Mercury is considered as a ubiquitous pollutant due to its versatile application in medicine, industry and cosmetics. Long non-coding RNAs (lncRNAs) are transcripts greater than 200 nt without protein-encoding function. However, little is known about the mechanism of heavy metals-induced non-coding RNA changes in aquatic organisms. To reveal the epigenetic mechanism of mercury toxicity in zebrafish embryos and explore novel specific mercury-toxicological biomarkers, several well-studied lncRNAs were screened by real-time PCR, and the spatial-temporal expression of lncRNAs biomarker was evaluated by in situ hybridization. The nerve system of zebrafish embryos were evaluated by detecting locomotor behavior and the expression of neuro-genes. We identified a mercury responsive lncRNA, metastasis-associated lung adenocarcinoma transcript 1 (malat1), among 5 candidate lncRNAs. HgCl2, MeHg, PbCl2, CdCl2 and K2CrO4 exposure assay showed that malat-1 was a mercury specific induced lncRNAs. Malat1 was highly expressed in the brain region, eyes and notochord of developing zebrafish embryos after exposure to mercury compounds. HgCl2 showed neurobehavior disturbance and changed neuro-genes expression pattern in zebrafish larval. This study provides a biological method to detect inorganic or organic mercury using malat1 as a novel biomarker of mercury contamination and also clues for the exploration of neurotoxicity mechanism of mercury compounds.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping