PUBLICATION

The effects of binary mixtures of estradiol and progesterone on transcriptional expression profiles of genes involved in hypothalamic-pituitary-gonadal axis and circadian rhythm signaling in embryonic zebrafish (Danio rerio)

Authors
Liang, Y.Q., Huang, G.Y., Zhen, Z., Tian, F., Hou, L., Lin, Z., Ying, G.G.
ID
ZDB-PUB-190314-16
Date
2019
Source
Ecotoxicology and environmental safety   174: 540-548 (Journal)
Registered Authors
Keywords
Circadian rhythm signaling, Estradiol, Hypothalamic-pituitary-gonadal axis, Mixture effects, Progesterone
MeSH Terms
  • Animals
  • Circadian Rhythm/drug effects*
  • Estradiol/toxicity*
  • Female
  • Gonads/drug effects
  • Gonads/metabolism
  • Hypothalamo-Hypophyseal System/drug effects*
  • Hypothalamo-Hypophyseal System/metabolism
  • Progesterone/toxicity*
  • Signal Transduction/drug effects
  • Transcriptome/drug effects*
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed
30865910 Full text @ Ecotoxicol. Environ. Saf.
Abstract
Natural and synthetic estrogens and progestins are present in the various aquatic environments, leading to potential exposure of aquatic organisms to their mixtures. However, very little is known about their combined effects in aquatic organisms. The aim of this study was to analyze the effects of binary mixtures of estradiol (E2) and progesterone (P4) by measuring transcriptional changes of up to 42 selected target genes related to hypothalamic-pituitary-gonadal axis and circadian rhythm signaling in zebrafish (Danio rerio) eleuthero-embryos. Zebrafish embryos were exposed to E2 and P4 alone or in combination at concentrations between 45 and 5217 ng L-1 for 96 h post fertilization (hpf). The results showed that P4 led to slight up-regulation of the cyp11a1, hsd17b3 and fshb transcripts, while a strong induction of cyp19a1b and lhb mRNA by E2 was observed. Also, cyp19a1b and lhb mRNAs expression were strongly up-regulated in the mixtures, which were the same to E2 alone. This finding suggests the mixture activity of E2 and P4 followed the independent action in zebrafish eleuthero-embryos. These transcriptional alterations may translate to adverse effects on sex differentiation and reproduction in fish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping