PUBLICATION
Characterization of the Vibrio cholerae phage shock protein response
- Authors
- DeAngelis, C.M., Nag, D., Withey, J.H., Matson, J.S.
- ID
- ZDB-PUB-190313-4
- Date
- 2019
- Source
- Journal of bacteriology 201(14): (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Cholera Toxin/genetics
- Cholera Toxin/metabolism*
- Vibrio cholerae/genetics*
- Vibrio cholerae/metabolism
- Heat-Shock Proteins/genetics
- Heat-Shock Proteins/metabolism*
- Gene Expression Regulation, Bacterial
- Mice
- Virulence
- Type II Secretion Systems/genetics
- Type II Secretion Systems/metabolism*
- Bacterial Proteins/genetics
- Bacterial Proteins/metabolism*
- Zebrafish/microbiology
- Animals
- Mucins/analysis
- PubMed
- 30858296 Full text @ J. Bacteriol.
Citation
DeAngelis, C.M., Nag, D., Withey, J.H., Matson, J.S. (2019) Characterization of the Vibrio cholerae phage shock protein response. Journal of bacteriology. 201(14):.
Abstract
The phage shock protein (Psp) system is a stress response pathway that senses and responds to inner membrane damage. The genetic components of the Psp system are present in several clinically relevant Gram-negative bacteria, including Vibrio cholerae However, most of the current knowledge about the Psp response stems from in vitro studies in Escherichia coli and Yersinia enterocolitica. In fact, the Psp response in V. cholerae has remained completely uncharacterized. In this study, we demonstrate that V. cholerae does have a functional Psp response system. We found that overexpression of GspD (EpsD), the type II secretion system secretin, induces the Psp response, whereas other V. cholerae secretins do not. In addition, we have identified several environmental conditions that induce this stress response. Our studies on the genetic regulation and induction of the Psp system in V. cholerae suggest that the key regulatory elements are conserved with those of other Gram-negative bacteria. While a psp null strain is fully capable of colonizing the infant mouse intestine, it exhibits a colonization defect in a zebrafish model, indicating that this response may be important for disease transmission in the environment. Overall, these studies provide an initial understanding of a stress response pathway that has not been previously investigated in V. choleraeImportanceVibrio cholerae leads a dual life cycle, as it can exist in the aquatic environment and colonize the human small intestine. In both life cycles, V. cholerae encounters a variety of stressful conditions, including fluctuating pH and temperature, and exposure to other agents that may negatively affect cell envelope homeostasis. The phage shock protein (Psp) response is required to sense and respond to such insults in other bacteria, but has remained unstudied in V. cholerae Interestingly, the Psp system has protein homologs, principally PspA, in a number of bacterial clades, as well as in archaea and plants. Therefore, our findings not only fill a gap in knowledge about an unstudied extracytoplasmic stress response in V. cholerae, but may have far-reaching implications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping