PUBLICATION

SLC39A6/ZIP6 is essential for zinc homeostasis and T-cell development in zebrafish

Authors
Zhao, L., Tan, J., Li, D., Jiang, L., Li, T., Yang, Y., Wang, G., Shang, Z., Wang, J., Zhou, J.
ID
ZDB-PUB-190312-2
Date
2019
Source
Biochemical and Biophysical Research Communications   511(4): 896-902 (Journal)
Registered Authors
Keywords
T lymphocyte, TALEN, Zebrafish, Zip6
MeSH Terms
  • Animals
  • Apoptosis
  • Cation Transport Proteins/genetics*
  • Cation Transport Proteins/metabolism
  • Cells, Cultured
  • Gene Deletion*
  • Gene Expression Regulation, Developmental
  • Homeostasis
  • T-Lymphocytes/cytology
  • T-Lymphocytes/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Zinc/metabolism*
PubMed
30851936 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Zinc (Zn) is an essential trace element that modulate innate and acquired immune responses, and its deficiency triggers lymphopenia. However, the precise mechanisms underlying zinc-mediated lymphocyte maintenance have not been well clarified. Here, we have successfully generated a zip6-null mutant zebrafish line using TALENs. The Zip6-null mutant zebrafish developed normally during gastrulation. Loss of zip6 in zebrafish resulted in significant T lymphocyte reduction and a decrease in intracellular Zn levels. And the zip6 deficiency increases caspase-related cell apoptosis in both zebrafish cells and human T cells. Our results suggest that ZIP6 plays a critical part in T cell development, and enhance our understanding of Zn homeostasis and immune system maintenance.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes