PUBLICATION
In vivo and in vitro analysis of starmaker activity in zebrafish otolith biomineralization
- Authors
- Kalka, M., Markiewicz, N., Ptak, M., Sone, E.D., Ożyhar, A., Dobryszycki, P., Wojtas, M.
- ID
- ZDB-PUB-190308-11
- Date
- 2019
- Source
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33(6): 6877-6886 (Journal)
- Registered Authors
- Keywords
- , calcium carbonate, phosphorylation, proteomics
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Biomineralization*
- Calcification, Physiologic*
- Calcium Carbonate/metabolism
- In Vitro Techniques
- Otolithic Membrane/growth & development
- Otolithic Membrane/physiology*
- Phosphorylation
- Proteome/analysis*
- Sequence Homology
- Zebrafish/physiology*
- Zebrafish Proteins/metabolism*
- PubMed
- 30840836 Full text @ FASEB J.
Citation
Kalka, M., Markiewicz, N., Ptak, M., Sone, E.D., Ożyhar, A., Dobryszycki, P., Wojtas, M. (2019) In vivo and in vitro analysis of starmaker activity in zebrafish otolith biomineralization. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 33(6):6877-6886.
Abstract
Otoliths are one of the biominerals whose formation is highly controlled by proteins. The first protein discovered to be involved in otolith biomineralization in zebrafish was starmaker (Stm). Previously, Stm was shown to be responsible for the preferential formation of aragonite, a polymorph of calcium carbonate, in otoliths. In this work, proteomic analysis of adult zebrafish otoliths was performed. Stm is the only highly phosphorylated protein found in our studies. Besides previously studied otolith proteins, we discovered several dozens of unknown proteins that reveal the likely mechanism of biomineralization. A comparison of aragonite and vaterite otoliths showed similarities in protein composition. We observed the presence of Stm in both types of otoliths. In vitro studies of 2 characteristic Stm fragments indicated that the DS-rich region has a special biomineralization activity, especially after phosphorylation.-Kalka, M., Markiewicz, N., Ptak, M., Sone, E. D., Ożyhar, A., Dobryszycki, P., Wojtas, M. In vivo and in vitro analysis of starmaker activity in zebrafish otolith biomineralization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping