PUBLICATION
Gene expression patterns and related enzymatic activities of detoxification and oxidative stress systems in zebrafish larvae exposed to the 2,4-dichlorophenoxyacetic acid herbicide
- Authors
- Gaaied, S., Oliveira, M., Le Bihanic, F., Cachot, J., Banni, M.
- ID
- ZDB-PUB-190303-11
- Date
- 2019
- Source
- Chemosphere 224: 289-297 (Journal)
- Registered Authors
- Keywords
- Biotransformation, Early life stages, Gene expression, Herbicide, Oxidative stress, Zebrafish
- MeSH Terms
-
- Oxidative Stress/drug effects*
- Oxidative Stress/genetics
- Transcriptome/drug effects*
- Herbicides/toxicity*
- Antioxidants/metabolism
- Larva/drug effects
- Larva/genetics
- Inactivation, Metabolic
- 2,4-Dichlorophenoxyacetic Acid/toxicity*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Animals
- Embryo, Nonmammalian*/drug effects
- Embryo, Nonmammalian*/enzymology
- PubMed
- 30825855 Full text @ Chemosphere
Citation
Gaaied, S., Oliveira, M., Le Bihanic, F., Cachot, J., Banni, M. (2019) Gene expression patterns and related enzymatic activities of detoxification and oxidative stress systems in zebrafish larvae exposed to the 2,4-dichlorophenoxyacetic acid herbicide. Chemosphere. 224:289-297.
Abstract
The present study aims to assess the effects of 2,4-D herbicide on biotransformation and oxidative stress status of zebrafish larvae. Animals were exposed to a range of sublethal concentrations (0.02-0.8 mg/L) and biomarkers at transcriptomic level and biochemical level were assessed. Chemical analysis with showed that the bioaccumulation of 2,4-D in 96 hpf zebrafish larvae were increased in a concentration-dependent manner. This herbicide induced significant effects at both gene expression and enzymatic activities levels after at 96 hpf. Results of mRNA expression showed a differential transcription regulation with all target genes depending on the tested concentrations. The mRNA level of gsr and cyp1a were up regulated at the highest dose of herbicide (0.8 mg/L). The gene expression of gstp1 showed an up regulation at lower dose (0.02 mg/L) and a down regulation at the highest dose (0.8 mg/L) of 2,4-D. A significant induction of EROD activity and inhibition of GST activity were noted in groups exposed to 0.8 mg/L of 2,4-D. Considering the antioxidant defenses, the activity of CAT was increased in larvae exposed to 0.8 mg/L of herbicide and GPx activity was induced at lower doses of 2,4-D (0.02 and 0.051 mg/L). Moreover, peroxidative damage, assessed as MDA content, was markedly increased in larvae exposed to high 2,4-D concentration. Overall, the present study data indicate that bioaccumulation of 2,4-D in 96 hpf zebrafish larvae and alterations in detoxification and oxidative stress related parameters, likely associated with ROS production, which may endanger the embryo-larval stages development of fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping