PUBLICATION
Fluid dynamics during bleb formation in migrating cells in vivo
- Authors
- Goudarzi, M., Boquet-Pujadas, A., Olivo-Marin, J.C., Raz, E.
- ID
- ZDB-PUB-190227-22
- Date
- 2019
- Source
- PLoS One 14: e0212699 (Journal)
- Registered Authors
- Goudarzi, Mohammad, Raz, Erez
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/embryology
- Animals, Genetically Modified/genetics
- Apoptosis/physiology
- Cell Movement/physiology*
- Cytokinesis/physiology*
- Cytoplasm/genetics
- Cytoplasm/metabolism*
- Germ Cells/cytology
- Germ Cells/metabolism*
- Models, Biological*
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 30807602 Full text @ PLoS One
Citation
Goudarzi, M., Boquet-Pujadas, A., Olivo-Marin, J.C., Raz, E. (2019) Fluid dynamics during bleb formation in migrating cells in vivo. PLoS One. 14:e0212699.
Abstract
Blebs are cellular protrusions observed in migrating cells and in cells undergoing spreading, cytokinesis, and apoptosis. Here we investigate the flow of cytoplasm during bleb formation and the concurrent changes in cell volume using zebrafish primordial germ cells (PGCs) as an in vivo model. We show that bleb inflation occurs concomitantly with cytoplasmic inflow into it and that during this process the total cell volume does not change. We thus show that bleb formation in primordial germ cells results primarily from redistribution of material within the cell rather than being driven by flow of water from an external source.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping