PUBLICATION
Toxic Effects of TiO₂ NPs on Zebrafish
- Authors
- Tang, T., Zhang, Z., Zhu, X.
- ID
- ZDB-PUB-190220-9
- Date
- 2019
- Source
- International Journal of Environmental Research and Public Health 16(4): (Journal)
- Registered Authors
- Keywords
- TiO2 NPs, oxidative damage, quantitative real-time reverse transcription PCR (qRT-PCR), zebrafish
- MeSH Terms
-
- Animals
- Adaptation, Physiological/drug effects
- Nanoparticles/chemistry
- Nanoparticles/toxicity*
- Oxidative Stress/drug effects
- Toxicity Tests, Acute
- Titanium/chemistry
- Titanium/toxicity*
- Zebrafish/growth & development
- Zebrafish/metabolism
- Zebrafish/physiology*
- Antioxidants/metabolism
- PubMed
- 30781732 Full text @ Int. J. Environ. Res. Public Health
Citation
Tang, T., Zhang, Z., Zhu, X. (2019) Toxic Effects of TiO₂ NPs on Zebrafish. International Journal of Environmental Research and Public Health. 16(4):.
Abstract
Titanium dioxide nanoparticles (TiO₂ NPs) have become a widely used nanomaterial due to the photocatalytic activity and absorption of ultraviolet light of specific wavelengths. This study investigated the toxic effects of rutile TiO₂ NPs on zebrafish by examining its embryos and adults. In the embryo acute toxicity test, exposure to 100 mg/L TiO₂ NPs didn't affect the hatching rate of zebrafish embryos, and there was no sign of deformity. In the adult toxicity test, the effects of TiO₂ NPs on oxidative damage in liver, intestine and gill tissue were studied. Enzyme linked immunosorbent assay (ELISA) and fluorescence-based quantitative real-time reverse transcription PCR (qRT-PCR) were used to detect the three antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathione S transferase (GSTs) in the above mentioned zebrafish organs at protein and gene levels. The results showed that long-term exposure to TiO₂ NPs can cause oxidative damage to organisms; and compared with the control group, the activity of the three kinds of enzyme declined somewhat at the protein level. In addition, long-term exposure to TiO₂ NPs could cause high expression of CAT, SOD and GSTs in three organs of adult zebrafish in order to counter the adverse reaction. The effects of long-term exposure to TiO₂ NPs to adult zebrafish were more obvious in the liver and gill.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping