PUBLICATION

Molecular and functional characterization of sirt4 and sirt6 in Megalobrama amblycephala under high glucose metabolism

Authors
Lin, S.T., Teng, L.Z., Lin, Y., Miao, L.H., Ge, X.P., Hao, J.Y., Huang, X., Liu, B.
ID
ZDB-PUB-190219-3
Date
2019
Source
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology   231: 87-97 (Journal)
Registered Authors
Keywords
Cloning, Functional characterization, High glucose metabolism, Sirtuin 4, Sirtuin 6
MeSH Terms
  • Base Sequence
  • Organ Specificity
  • Glucose/metabolism
  • Glucose/pharmacology*
  • Animals
  • Cyprinidae/genetics*
  • Cyprinidae/metabolism*
  • Sirtuins/chemistry
  • Sirtuins/genetics*
  • Sirtuins/metabolism*
  • Amino Acid Sequence
  • Gene Expression Regulation/drug effects
  • Dose-Response Relationship, Drug
(all 13)
PubMed
30772485 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Abstract
We analyzed the sequences of sirt4 and sirt6 and their changes in expression after oral glucose administration in blunt snout bream (Megalobrama amblycephala). We cloned sirt4 and sirt6 and found that their full-length cDNA sequences were 1530 bp and 1723 bp, respectively; their amino acid sequences were 93% and 92% identical to Danio rerio. Megalobrama amblycephala were fed a high glucose solution (3 g/kg). Normally, sirt4 expression is higher in spleen, intestine, and gill (P < .05), and sirt6 expression is higher in intestine and gill (P < .05). After oral glucose administration, sirt4 and sirt6 expression increased in liver and gill, and sirt4 expression increased in intestine at 0.5 h (P < .05). In contrast, sirt4 in kidney and sirt6 in head kidney were downregulated at 1 h (P < .05). Expression of sirt4 was upregulated in brain, head kidney, spleen, muscle, and liver 2 h, 4 h, 4 h/24 h, 8 h, and 24 h, respectively (P < .05). Expression of sirt4 was downregulated in kidney at 8 h-48 h (P < .05). Expression of sirt6 was upregulated in intestine, liver, muscle, kidney, and spleen at 4 h/24 h, 8 h/24 h, 12 h, 12 h, and 24 h, respectively (P < .05). We report that sirt4 and sirt6 are highly conserved in evolution and exhibit tissue-specific expression profiles. We demonstrate that the expression of sirt4 and sirt6 are tissue-specific, and depend upon tissue-specific responses to glucose metabolism.
Genes / Markers
Figures
No images available
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
No data available
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Lin, S.T., Teng, L.Z., Lin, Y., Miao, L.H., Ge, X.P., Hao, J.Y., Huang, X., Liu, B. (2019) Molecular and functional characterization of sirt4 and sirt6 in Megalobrama amblycephala under high glucose metabolism. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 231:87-97.