PUBLICATION

Dynamics of cardiomyocyte transcriptome and chromatin landscape demarcates key events of heart development

Authors
Pawlak, M., Kedzierska, K.Z., Migdal, M., Nahia, K.A., Ramilowski, J.A., Bugajski, L., Hashimoto, K., Marconi, A., Piwocka, K., Carninci, P., Winata, C.L.
ID
ZDB-PUB-190215-7
Date
2019
Source
Genome research   29(3): 506-519 (Journal)
Registered Authors
Pawlak, Michal, Winata, Cecilia Lanny
Keywords
none
Datasets
GEO:GSE120238, GEO:GSE120236, GEO:GSE120237
MeSH Terms
  • Animals
  • Cells, Cultured
  • Chromatin/genetics
  • Chromatin Assembly and Disassembly*
  • Gene Expression Regulation, Developmental*
  • Gene Regulatory Networks
  • Heart/growth & development*
  • Myocytes, Cardiac/metabolism*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Transcriptome*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
30760547 Full text @ Genome Res.
Abstract
Organogenesis involves dynamic regulation of gene transcription and complex multipathway interactions. Despite our knowledge of key factors regulating various steps of heart morphogenesis, considerable challenges in understanding its mechanism still exist because little is known about their downstream targets and interactive regulatory network. To better understand transcriptional regulatory mechanism driving heart development and the consequences of its disruption in vivo, we performed time-series analyses of the transcriptome and genome-wide chromatin accessibility in isolated cardiomyocytes (CMs) from wild-type zebrafish embryos at developmental stages corresponding to heart tube morphogenesis, looping, and maturation. We identified genetic regulatory modules driving crucial events of heart development that contained key cardiac TFs and are associated with open chromatin regions enriched for DNA sequence motifs belonging to the family of the corresponding TFs. Loss of function of cardiac TFs Gata5, Tbx5a, and Hand2 affected the cardiac regulatory networks and caused global changes in chromatin accessibility profile, indicating their role in heart development. Among regions with differential chromatin accessibility in mutants were highly conserved noncoding elements that represent putative enhancers driving heart development. The most prominent gene expression changes, which correlated with chromatin accessibility modifications within their proximal promoter regions, occurred between heart tube morphogenesis and looping, and were associated with metabolic shift and hematopoietic/cardiac fate switch during CM maturation. Our results revealed the dynamic regulatory landscape throughout heart development and identified interactive molecular networks driving key events of heart morphogenesis.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping