PUBLICATION
Visualizing the Balbiani Body in Zebrafish Oocytes
- Authors
- Lee, K.L., Marlow, F.L.
- ID
- ZDB-PUB-190210-6
- Date
- 2019
- Source
- Methods in molecular biology (Clifton, N.J.) 1920: 277-293 (Chapter)
- Registered Authors
- Marlow, Florence
- Keywords
- Balbiani body, Buckyball, Histology, Immunohistochemistry, Microscopy, Mitochondria, Oocyte, Primary oocytes, RNA and protein localization
- MeSH Terms
-
- Animals
- Biomarkers
- Endoplasmic Reticulum/metabolism
- Female
- Fluorescent Antibody Technique
- Immunohistochemistry
- In Situ Hybridization
- Mitochondria/metabolism
- Molecular Imaging/methods*
- Oocytes/metabolism*
- Oogenesis*
- RNA/genetics
- RNA/metabolism
- Zebrafish*
- PubMed
- 30737697 Full text @ Meth. Mol. Biol.
Citation
Lee, K.L., Marlow, F.L. (2019) Visualizing the Balbiani Body in Zebrafish Oocytes. Methods in molecular biology (Clifton, N.J.). 1920:277-293.
Abstract
Approaches to visualize the Balbiani body of zebrafish primary oocytes using protein, RNA, and mitochondrial markers are described. The method involves isolation, histology, staining, and microscopic examination of early zebrafish oocytes. These techniques can be applied to visualize gene products that are localized to the Balbiani body, and when applied to mutants can be used to decipher molecular and genetic pathways acting in Balbiani body development in early oocytes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping