PUBLICATION
Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping
- Authors
- Tabor, K.M., Marquart, G.D., Hurt, C., Smith, T.S., Geoca, A.K., Bhandiwad, A.A., Subedi, A., Sinclair, J.L., Rose, H.M., Polys, N.F., Burgess, H.A.
- ID
- ZDB-PUB-190209-18
- Date
- 2019
- Source
- eLIFE 8: (Journal)
- Registered Authors
- Burgess, Harold, Marquart, Gregory, Subedi, Abhi, Tabor, Kathryn
- Keywords
- neuroscience, zebrafish
- MeSH Terms
-
- Animals
- Brain Mapping/methods*
- Integrases/genetics
- Animals, Genetically Modified/genetics
- DNA-Binding Proteins/genetics
- PubMed
- 30735129 Full text @ Elife
Abstract
Decoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using intersectional genetic methods which restrict reporter expression to cells that co-express multiple drivers, such as Gal4 and Cre. To facilitate intersectional targeting in zebrafish, we have generated more than 50 new Cre lines, and co-registered brain expression images with the Zebrafish Brain Browser, a cellular resolution atlas of 264 transgenic lines. Lines labeling neurons of interest can be identified using a web-browser to perform a 3D spatial search (zbbrowser.com). This resource facilitates the design of intersectional genetic experiments and will advance a wide range of precision circuit-mapping studies.
Errata / Notes
See directly submitted expression data. May load slowly.
Genes / Markers
Figure Gallery (6 images)
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping