PUBLICATION
Expression and localization of grass carp pkc-θ (protein kinase C theta) gene after its activation
- Authors
- Mehjabin, R., Chen, L., Huang, R., Zhu, D., Yang, C., Li, Y., Liao, L., He, L., Zhu, Z., Wang, Y.
- ID
- ZDB-PUB-190205-12
- Date
- 2019
- Source
- Fish & shellfish immunology 87: 788-795 (Journal)
- Registered Authors
- Zhu, Zuoyan
- Keywords
- Grass carp, Grass carp reovirus (GCRV), Host immune defences, Protein kinase C (PKC)
- MeSH Terms
-
- Sequence Alignment/veterinary
- Reoviridae/physiology
- Carps/genetics*
- Carps/immunology*
- Reoviridae Infections/immunology
- PubMed
- 30716520 Full text @ Fish Shellfish Immunol.
Abstract
Haemorrhagic disease caused by grass carp reovirus (GCRV) can result in large-scale death of young grass carp, leading to irreparable economic losses that seriously affect large-scale breeding. Protein kinase C (PKC, also known as PRKC) represents a family of serine/threonine protein kinases that includes multiple isozymes in many species. Among these, PKC-θ (PKC theta, also written as PRKCQ) is a novel isoform, mainly expressed in T cells, that is known to be involved in immune system function in mammals. To date, no research on immunological functions of fish Pkc-θ has been reported. To address this issue, we cloned the grass carp pkc-θ gene. Phylogenetic and syntenic analysis showed that this gene is the most evolutionarily conserved relative to zebrafish. Real-time quantitative PCR (RT-qPCR) indicated that pkc-θ was expressed at high levels in the gills and spleen of healthy grass carp. Infection with GCRV down regulated pkc-θ expression in the gills and spleen. Gene products that function upstream and downstream of pkc-θ were up regulated in the gill, but were down-regulated in the spleen. These results suggest that direct or indirect targeting of pkc-θ by GCRV may help the virus evade host immune defences in the spleen. Phorbol ester (PMA) treatment of Jurkat T cells induced translocation of grass carp Pkc-θ from the cytoplasm to the plasma membrane. This response to PMA suggests evolutionary conservation of an immune response function in fish Pkc-θ, as well as conservation of its sequence and structural domains. This study expanded our knowledge of the fish PKC gene family, and explored the role of pkc-θ in function of the grass carp immune system, providing new insights which may facilitate further studies of its biological functions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping