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ZFIN ID: ZDB-PUB-190205-12
Expression and localization of grass carp pkc-θ (protein kinase C theta) gene after its activation
Mehjabin, R., Chen, L., Huang, R., Zhu, D., Yang, C., Li, Y., Liao, L., He, L., Zhu, Z., Wang, Y.
Date: 2019
Source: Fish & shellfish immunology   87: 788-795 (Journal)
Registered Authors: Zhu, Zuoyan
Keywords: Grass carp, Grass carp reovirus (GCRV), Host immune defences, Protein kinase C (PKC)
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carps/genetics*
  • Carps/immunology*
  • Fish Diseases/immunology*
  • Fish Proteins/chemistry
  • Fish Proteins/genetics
  • Fish Proteins/immunology
  • Gene Expression Profiling/veterinary
  • Gene Expression Regulation/immunology*
  • Immunity, Innate/genetics*
  • Protein Kinase C-theta/chemistry
  • Protein Kinase C-theta/genetics*
  • Protein Kinase C-theta/immunology*
  • Random Allocation
  • Reoviridae/physiology
  • Reoviridae Infections/immunology
  • Reoviridae Infections/veterinary
  • Sequence Alignment/veterinary
PubMed: 30716520 Full text @ Fish Shellfish Immunol.
Haemorrhagic disease caused by grass carp reovirus (GCRV) can result in large-scale death of young grass carp, leading to irreparable economic losses that seriously affect large-scale breeding. Protein kinase C (PKC, also known as PRKC) represents a family of serine/threonine protein kinases that includes multiple isozymes in many species. Among these, PKC-θ (PKC theta, also written as PRKCQ) is a novel isoform, mainly expressed in T cells, that is known to be involved in immune system function in mammals. To date, no research on immunological functions of fish Pkc-θ has been reported. To address this issue, we cloned the grass carp pkc-θ gene. Phylogenetic and syntenic analysis showed that this gene is the most evolutionarily conserved relative to zebrafish. Real-time quantitative PCR (RT-qPCR) indicated that pkc-θ was expressed at high levels in the gills and spleen of healthy grass carp. Infection with GCRV down regulated pkc-θ expression in the gills and spleen. Gene products that function upstream and downstream of pkc-θ were up regulated in the gill, but were down-regulated in the spleen. These results suggest that direct or indirect targeting of pkc-θ by GCRV may help the virus evade host immune defences in the spleen. Phorbol ester (PMA) treatment of Jurkat T cells induced translocation of grass carp Pkc-θ from the cytoplasm to the plasma membrane. This response to PMA suggests evolutionary conservation of an immune response function in fish Pkc-θ, as well as conservation of its sequence and structural domains. This study expanded our knowledge of the fish PKC gene family, and explored the role of pkc-θ in function of the grass carp immune system, providing new insights which may facilitate further studies of its biological functions.