PUBLICATION
Adverse effects of p-TSA-doped polypyrrole particulate exposure during zebrafish (Danio rerio) development
- Authors
- Costa, K.M., Pereira, T.C.B., Valente, C.A., Pissinate, K., Soares, J.C., Cruz, F.F., Corte, T.W.F., Machado, P., Basso, N.R.S., Bogo, M.R.
- ID
- ZDB-PUB-190204-6
- Date
- 2019
- Source
- Colloids and surfaces. B, Biointerfaces 177: 58-67 (Journal)
- Registered Authors
- Keywords
- Behavior, Nanomaterials, Nanotoxicity, PPy, PPy/p-TSA, Zebrafish development
- MeSH Terms
-
- Dose-Response Relationship, Drug
- Larva/drug effects*
- Surface Properties
- Polymers/chemistry
- Polymers/pharmacology*
- Animals
- Particle Size
- Molecular Structure
- Pyrroles/chemistry
- Pyrroles/pharmacology*
- Benzenesulfonates/chemistry
- Benzenesulfonates/pharmacology*
- Zebrafish
- Embryo, Nonmammalian/drug effects*
- PubMed
- 30711760 Full text @ Colloids Surf. B Biointerfaces
Citation
Costa, K.M., Pereira, T.C.B., Valente, C.A., Pissinate, K., Soares, J.C., Cruz, F.F., Corte, T.W.F., Machado, P., Basso, N.R.S., Bogo, M.R. (2019) Adverse effects of p-TSA-doped polypyrrole particulate exposure during zebrafish (Danio rerio) development. Colloids and surfaces. B, Biointerfaces. 177:58-67.
Abstract
Nanomaterials have been attracting attention due to the wide range of applications in nanomedicine. Polypyrrole (PPy), a conductive polymer, has been employed in the biomedical field due to its stimulus-responsive properties, although in vivo studies to assess its potential undesirable effects are limited. This study evaluated the effects of PPy doped with p-toluene sulfonic acid ((p-TSA); PPy/p-TSA) exposure (at 25, 100, 250 and 500 μg/mL) during six consecutive days on mortality, hatching, spontaneous movement, heart rate, morphology and locomotion behavior of zebrafish embryos/larvae. Additionally, PPy/p-TSA envelopment of developing embryo chorions and gene expression of a hypoxia-related marker in this context were also evaluated. No significant mortality was found; however, altered heart rate and early hatching was identified in all exposed groups at 48 hours post-fertilization (hpf). Surprisingly, with the 500 μg/mL dose, hatching initiated as early as 24 hpf. PPy/p-TSA adhered to and enveloped the chorion of embryos in a time- and dose-dependent fashion; morphological changes in body length and ocular distance were found with higher concentrations. PPy/p-TSA-exposed animals showed locomotor behavioral alterations compatible with hypoactivity. A significant increase in the turn angle with a concomitant reduction in meander was also verified at higher concentrations. Taken together, these results emphasize the adverse effects of PPy/p-TSA on zebrafish development and behavior. Some effects of PPy/p-TSA exposure were dose-dependent, and indicate specific adverse effects of PPy/p-TSA on zebrafish development and behavior.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping