PUBLICATION
Evaluation of the developmental toxicity of 2,7-dibromocarbazole to zebrafish based on transcriptomics assay
- Authors
- Ji, C., Yan, L., Chen, Y., Yue, S., Dong, Q., Chen, J., Zhao, M.
- ID
- ZDB-PUB-190203-18
- Date
- 2019
- Source
- Journal of hazardous materials 368: 514-522 (Journal)
- Registered Authors
- Keywords
- 2,7-Dibromocarbazole, Cardiac teratogenic effect, Polyhalogenated carbazoles, Transcriptomics, Transgenic zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Carbazoles/toxicity*
- Gene Expression Regulation, Developmental/drug effects
- Heart/drug effects
- Heart/growth & development
- Larva/drug effects
- Larva/genetics
- Larva/growth & development
- Teratogens/toxicity*
- Transcriptome/drug effects
- Zebrafish*/abnormalities
- Zebrafish*/genetics
- Zebrafish Proteins/genetics
- PubMed
- 30710780 Full text @ J. Hazard. Mater.
Citation
Ji, C., Yan, L., Chen, Y., Yue, S., Dong, Q., Chen, J., Zhao, M. (2019) Evaluation of the developmental toxicity of 2,7-dibromocarbazole to zebrafish based on transcriptomics assay. Journal of hazardous materials. 368:514-522.
Abstract
Polyhalogenated carbazoles (PHCZs), which have the similar structure of dioxin, have been reported ubiquitous in the environments and drawn wide concerns. However, their potential ecological and health risks are still poorly understood. Here, wildtype zebrafish embryos were used to evaluate the environmental risks of 2,7-dibromocarbazole (2,7-DBCZ), 3,6-dibromocarbazole (3,6-DBCZ), and 3,6-dichlorocarbazole (3,6-DCCZ). 2,7-DBCZ was the most toxic compound with the 96-h LC50 value of 581.8 ± 29.3 μg·L-1 and the EC50 value of 201.5 ± 6.5 μg·L-1 for pericardial edema. The teratogenic effects of 2,7-DBCZ were tested using transgenic zebrafish larvae. The transcriptomic analysis revealed that 90 genes in zebrafish expressed differently after exposure to 2,7-DBCZ, and many pathways were related to aryl hydrocarbon receptor (AhR) activation. The qRT-PCR also showed that expression levels of AhR1 and CYP1 A in zebrafish were significantly up-regulated after exposure to 2,7-DBCZ. In conclusion, 2,7-DBCZ exhibited more potent toxicity and cardiac teratogenic effects, and presented developmental toxicity partially consistent with AhR activation. Our results will be of great help to the risk assessment and regulation-making of PHCZs. Meanwhile, further studies should be promoted to illustrate the potential mechanism between PHCZs and AhR in the near future.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping