ZFIN ID: ZDB-PUB-190131-4
ARGLU1 is a transcriptional coactivator and splicing regulator important for stress hormone signaling and development
Magomedova, L., Tiefenbach, J., Zilberman, E., Le Billan, F., Voisin, V., Saikali, M., Boivin, V., Robitaille, M., Gueroussov, S., Irimia, M., Ray, D., Patel, R., Xu, C., Jeyasuria, P., Bader, G.D., Hughes, T.R., Morris, Q.D., Scott, M.S., Krause, H., Angers, S., Blencowe, B.J., Cummins, C.L.
Date: 2019
Source: Nucleic acids research   47(6): 2856-2870 (Journal)
Registered Authors: Irimia, Manuel, Krause, Henry, Tiefenbach, Jens
Keywords: none
MeSH Terms:
  • Alternative Splicing/drug effects
  • Alternative Splicing/genetics
  • Animals
  • Animals, Genetically Modified
  • Cells, Cultured
  • Embryo, Nonmammalian
  • Embryonic Development*/drug effects
  • Embryonic Development*/genetics
  • Glucocorticoids/metabolism
  • Glucocorticoids/pharmacology*
  • HEK293 Cells
  • Humans
  • Intracellular Signaling Peptides and Proteins/physiology*
  • Mice
  • Mice, Inbred C57BL
  • Neurogenesis/drug effects
  • Neurogenesis/genetics
  • RNA Splicing/drug effects
  • RNA Splicing/genetics*
  • Signal Transduction/drug effects
  • Signal Transduction/genetics
  • Stress, Physiological/drug effects
  • Stress, Physiological/genetics
  • Trans-Activators/physiology
  • Transcriptional Activation/drug effects
  • Transcriptional Activation/genetics*
  • Zebrafish
PubMed: 30698747 Full text @ Nucleic Acids Res.
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ABSTRACT
Stress hormones bind and activate the glucocorticoid receptor (GR) in many tissues including the brain. We identified arginine and glutamate rich 1 (ARGLU1) in a screen for new modulators of glucocorticoid signaling in the CNS. Biochemical studies show that the glutamate rich C-terminus of ARGLU1 coactivates multiple nuclear receptors including the glucocorticoid receptor (GR) and the arginine rich N-terminus interacts with splicing factors and binds to RNA. RNA-seq of neural cells depleted of ARGLU1 revealed significant changes in the expression and alternative splicing of distinct genes involved in neurogenesis. Loss of ARGLU1 is embryonic lethal in mice, and knockdown in zebrafish causes neurodevelopmental and heart defects. Treatment with dexamethasone, a GR activator, also induces changes in the pattern of alternatively spliced genes, many of which were lost when ARGLU1 was absent. Importantly, the genes found to be alternatively spliced in response to glucocorticoid treatment were distinct from those under transcriptional control by GR, suggesting an additional mechanism of glucocorticoid action is present in neural cells. Our results thus show that ARGLU1 is a novel factor for embryonic development that modulates basal transcription and alternative splicing in neural cells with consequences for glucocorticoid signaling.
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