PUBLICATION
Analysis of cilia dysfunction phenotypes in zebrafish embryos depleted of Origin recognition complex factors
- Authors
- Maerz, L.D., Casar Tena, T., Gerhards, J., Donow, C., Jeggo, P.A., Philipp, M.
- ID
- ZDB-PUB-190131-3
- Date
- 2019
- Source
- European journal of human genetics : EJHG 27(5): 772-782 (Journal)
- Registered Authors
- Philipp, Melanie
- Keywords
- none
- MeSH Terms
-
- Animals
- Cilia/metabolism*
- Ciliopathies/genetics
- Embryo, Nonmammalian/metabolism*
- Organogenesis
- Origin Recognition Complex/metabolism*
- Phenotype
- Zebrafish/embryology*
- Zebrafish Proteins/metabolism*
- PubMed
- 30696958 Full text @ Eur. J. Hum. Genet.
Citation
Maerz, L.D., Casar Tena, T., Gerhards, J., Donow, C., Jeggo, P.A., Philipp, M. (2019) Analysis of cilia dysfunction phenotypes in zebrafish embryos depleted of Origin recognition complex factors. European journal of human genetics : EJHG. 27(5):772-782.
Abstract
Meier-Gorlin syndrome (MGS) is a rare, congenital primordial microcephalic dwarfism disorder. MGS is caused by genetic variants of components of the origin recognition complex (ORC) consisting of ORC1-6 and the pre-replication complex, which together enable origin firing and hence genome replication. In addition, ORC1 has previously been shown to play a role in ciliogenesis. Here, we extend this work and investigate the function of ORC1 and two other members of the complex on cilia at an organismal level. Knockdown experiments in zebrafish confirmed the impact of ORC1 on cilia. ORC1-deficiency confers defects anticipated to arise from impaired cilia function such as formation of oedema, kidney cysts, curved bodies and left-right asymmetry defects. We found ORC1 furthermore required for cilium formation in zebrafish and demonstrate that ciliopathy phenotypes in ORC1-depleted zebrafish could not be rescued by reconstitution with ORC1 bearing a genetic variant previously identified in MGS patients. Loss-of-function of Orc4 and Orc6, respectively, conferred similar ciliopathy phenotypes and cilium shortening in zebrafish, suggesting that several, if not all, components of the ORC regulate ciliogenesis downstream to or in addition to their canonical function in replication initiation. This study presents the first in vivo evidence of an influence of the MGS genes of the ORC family on cilia, and consolidates the possibility that cilia dysfunction could contribute to the clinical manifestation of ORC-deficient MGS.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping