Photoreceptor cell development requires prostaglandin signaling in the zebrafish retina
- Li, W., Jin, D., Zhong, T.P.
- Biochemical and Biophysical Research Communications 510(2): 230-235 (Journal)
- Registered Authors
- Jin, Daqing, Zhong, Tao P.
- Cilia, Photoreceptor, Prostaglandin, Retina, Zebrafish, ptgs
- MeSH Terms
- Cell Differentiation
- Cyclooxygenase 1/genetics
- Cyclooxygenase 2/genetics
- Photoreceptor Cells/metabolism*
- Photoreceptor Cells, Vertebrate/metabolism*
- Signal Transduction
- Zebrafish Proteins/genetics*
- 30685088 Full text @ Biochem. Biophys. Res. Commun.
Li, W., Jin, D., Zhong, T.P. (2019) Photoreceptor cell development requires prostaglandin signaling in the zebrafish retina. Biochemical and Biophysical Research Communications. 510(2):230-235.
Photoreceptor cells are highly specialized sensory neurons capable of visual phototransduction. The connecting cilia in the retinal photoreceptors link the inner segment to the outer segment and mediate the transport of opsins in vision. Although our previous study demonstrates that Prostaglandin E2 (PGE2) signaling is required for ciliogenesis in zebrafish, its roles in retinal ciliogenesis and photoreceptor cell development remain unknown. Here, we investigated the function of prostaglandin signaling in retina and photoreceptor cell development. We generated zebrafish mutations in prostaglandin endoperoxide synthase 1 (PTGS1) and prostaglandin endoperoxide synthase 2 (PTGS2), two rate-limiting enzymes responsible for prostaglandin production. We found that ptgs2b knockdown in ptgs1-/-ptgs2a-/- double mutants significantly reduced the length of connecting cilia and resulted in severe defects in photoreceptor cell differentiation. Furthermore, mutation in PGE2 transporter Leakytail (Lkt)/ATP-binding cassette transporter 4 (ABCC4) exhibited loss of connecting cilia and outer segment in photoreceptor cells, leading to mislocalization of opsins in the cell bodies of photoreceptors. Together, our findings suggest that PGE2 production and transport are crucial for connecting cilia formation and photoreceptor cell development.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes