PUBLICATION
Knockout of Nr2e3 prevents rod photoreceptor differentiation and leads to selective L-/M-cone photoreceptor degeneration in zebrafish
- Authors
- Xie, S., Han, S., Qu, Z., Liu, F., Li, J., Yu, S., Reilly, J., Tu, J., Liu, X., Lu, Z., Hu, X., Yimer, T.A., Qin, Y., Huang, Y., Lv, Y., Jiang, T., Shu, X., Tang, Z., Jia, H., Wong, F., Liu, M.
- ID
- ZDB-PUB-190127-20
- Date
- 2019
- Source
- Biochimica et biophysica acta. Molecular basis of disease 1865(6): 1273-1283 (Journal)
- Registered Authors
- Han, Shanshan, Huang, Yuwen, Hu, Xuebin, Jia, Haibo, Li, Jingzhen, Liu, Fei, Liu, Mugen, Liu, Xiliang, Lu, Zhaojing, Qin, Yayun, Qu, Zhen, Yu, Shanshan
- Keywords
- CRISPR, Degeneration, Differentiation, Nr2e3, Photoreceptor, Zebrafish
- MeSH Terms
-
- Animals
- Base Sequence
- CRISPR-Cas Systems
- Cell Differentiation/genetics*
- Gene Knockout Techniques
- HEK293 Cells
- Humans
- Microscopy, Electron, Transmission
- Mutation*
- Receptors, Cytoplasmic and Nuclear/genetics*
- Receptors, Cytoplasmic and Nuclear/metabolism
- Retina/embryology
- Retina/growth & development
- Retina/ultrastructure
- Retinal Cone Photoreceptor Cells/metabolism
- Retinal Cone Photoreceptor Cells/pathology*
- Retinal Degeneration/genetics*
- Retinal Degeneration/metabolism
- Retinal Degeneration/pathology
- Retinal Rod Photoreceptor Cells/cytology
- Retinal Rod Photoreceptor Cells/metabolism*
- Zebrafish
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 30684641 Full text @ BBA Molecular Basis of Disease
Citation
Xie, S., Han, S., Qu, Z., Liu, F., Li, J., Yu, S., Reilly, J., Tu, J., Liu, X., Lu, Z., Hu, X., Yimer, T.A., Qin, Y., Huang, Y., Lv, Y., Jiang, T., Shu, X., Tang, Z., Jia, H., Wong, F., Liu, M. (2019) Knockout of Nr2e3 prevents rod photoreceptor differentiation and leads to selective L-/M-cone photoreceptor degeneration in zebrafish. Biochimica et biophysica acta. Molecular basis of disease. 1865(6):1273-1283.
Abstract
Mutations in the photoreceptor cell-specific nuclear receptor gene Nr2e3 increased the number of S-cone photoreceptors in human and murine retinas and led to retinal degeneration that involved photoreceptor and non-photoreceptor cells. The mechanisms underlying these complex phenotypes remain unclear. In the hope of understanding the precise role of Nr2e3 in photoreceptor cell fate determination and differentiation, we generated a line of Nr2e3 knockout zebrafish using CRISPR technology. In these Nr2e3-null animals, rod precursors undergo terminal mitoses but fail to differentiate as rods. Rod-specific genes are not expressed and the outer segment (OS) fails to form. Formation and differentiation of cone photoreceptors is normal. Specifically, there is no increase in the number of UV-cone or S-cone photoreceptors. Laminated retinal structure is maintained. After normal development, L-/M-cones selectively degenerate, with progressive shortening of OS that starts at age 1 month. The amount of cone phototransduction proteins is concomitantly reduced, whereas UV- and S-cones have normal OS lengths even at age 10 months. In vitro studies show Nr2e3 synergizes with Crx and Nrl to enhance rhodopsin gene expression. Nr2e3 does not affect cone opsin expression. Our results extend the knowledge of Nr2e3's roles and have specific implications for the interpretation of the phenotypes observed in human and murine retinas. Furthermore, our model may offer new opportunities in finding treatments for enhanced S-cone syndrome (ESCS) and other retinal degenerative diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping