PUBLICATION
Zebrafish embryogenesis - A framework to study regulatory RNA elements in development and disease
- Authors
- Zaucker, A., Kumari, P., Sampath, K.
- ID
- ZDB-PUB-190120-5
- Date
- 2019
- Source
- Developmental Biology 457(2): 172-180 (Review)
- Registered Authors
- Kumari, Pooja, Sampath, Karuna, Zaucker, Andreas
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/embryology
- Embryonic Development/genetics
- Gene Expression Regulation, Developmental/genetics*
- RNA/genetics
- RNA Processing, Post-Transcriptional/genetics*
- Regulatory Sequences, Nucleic Acid/genetics*
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 30659794 Full text @ Dev. Biol.
Citation
Zaucker, A., Kumari, P., Sampath, K. (2019) Zebrafish embryogenesis - A framework to study regulatory RNA elements in development and disease. Developmental Biology. 457(2):172-180.
Abstract
Post-transcriptional gene regulation through the recognition of specific elements in mRNAs is an important determinant of gene expression. The cis elements are recognised by RNA binding proteins (RBPs) and/or small non-coding RNAs, which then orchestrate a range of processes such as mRNA localization, translational control, and degradation. RNA regulation is critical for development and disruptions in regulatory mechanisms can cause disease. While mutations in numerous RBPs have been linked to diseases in humans, the contribution of mutations in RNA elements to disease manifestation is largely unknown. Danio rerio (zebrafish), a fish model is a widely used vertebrate system to study development and disease. Here, we describe how state-of-the-art genomics tools combined with in vivo functional studies in zebrafish have facilitated the discovery of RNA elements, many of which are functionally conserved. We also highlight the potential of zebrafish to model human diseases and for drug discovery.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping