PUBLICATION

A ribosomal DNA-hosted microRNA regulates zebrafish embryonic angiogenesis

Authors
Shi, Y., Duan, X., Xu, G., Wang, X., Wei, G., Dong, S., Xie, G., Liu, D.
ID
ZDB-PUB-190119-2
Date
2019
Source
Angiogenesis   22(2): 211-221 (Journal)
Registered Authors
Liu, Dong, Shi, Yunwei
Keywords
Angiogenesis, Endothelial cells, MiRNA, Ribosomal DNA, Zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cloning, Molecular
  • DNA, Ribosomal/genetics*
  • Embryo, Nonmammalian/blood supply
  • Embryonic Development/genetics
  • Endothelium, Vascular/embryology*
  • Endothelium, Vascular/physiology
  • MicroRNAs/genetics
  • MicroRNAs/physiology*
  • Neovascularization, Physiologic/genetics*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed
30656567 Full text @ Angiogenesis
Abstract
MicroRNAs (miRNAs) are single-stranded small non-coding RNAs, generally 18-25 nucleotides in length, that act as repressors of gene expression. miRNAs are encoded by independent genes or processed from a variety of different RNA species. So far, there is no evidence showing that the ribosomal DNA-hosted microRNA is implicated in vertebrate development. Currently, we found a highly expressed small RNA hosted in ribosomal DNA was predicted as a novel miRNA, named miR-ntu1, in zebrafish endothelial cells by deep sequencing analysis. The miRNA was validated by custom-designed Taqman PCR, Northern Blot, and in silico analysis. Furthermore, we demonstrated that miR-ntu1 played a crucial role in zebrafish angiogenesis via modulation of Notch signaling. Our findings provide a notable case that a miRNA hosted in ribosomal DNA is involved in vertebrate development.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes