PUBLICATION

Light-activated Frizzled7 reveals a permissive role of non-canonical Wnt signaling in mesendoderm cell migration

Authors
?apek, D., Smutny, M., Tichy, A.M., Morri, M., Janovjak, H., Heisenberg, C.P.
ID
ZDB-PUB-190117-5
Date
2019
Source
eLIFE   8: (Journal)
Registered Authors
Heisenberg, Carl-Philipp, Smutny, Michael
Keywords
cell biology, developmental biology, zebrafish
MeSH Terms
  • Wnt Signaling Pathway/radiation effects*
  • Stem Cells/cytology
  • Stem Cells/radiation effects
  • Receptors, Cell Surface/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Light*
  • Mutation/genetics
  • Animals, Genetically Modified
  • Endoderm/cytology*
  • Zebrafish Proteins/metabolism*
  • Animals
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/radiation effects
  • Phenotype
  • Cell Movement/radiation effects*
  • Mesoderm/cytology*
(all 17)
PubMed
30648973 Full text @ Elife
Abstract
Non-canonical Wnt-signaling plays a central role for coordinated cell polarization and directed migration in metazoan development. While spatiotemporally restricted activation of non-canonical Wnt-signaling drives cell polarization in epithelial tissues, it remains unclear whether such instructive activity is also critical for directed mesenchymal cell migration. Here, we developed a light-activated version of the non-canonical Wnt receptor Frizzled 7 (Fz7) to analyze how restricted activation of non-canonical Wnt-signaling affects directed anterior axial mesendoderm (prechordal plate, ppl) cell migration within the zebrafish gastrula. We found that Fz7 signaling is required for ppl cell protrusion formation and migration, and that spatiotemporally restricted ectopic activation is capable of redirecting their migration. Finally, we show that uniform activation of Fz7 signaling in ppl cells fully rescues defective directed cell migration in fz7 mutant embryos. Together, our findings reveal that in contrast to the situation in epithelial cells, non-canonical Wnt-signaling functions permissively rather than instructively in directed mesenchymal cell migration during gastrulation.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
e3
    		Point Mutation
    e120TgTransgenic Insertion
      hu3495
        		Point Mutation
        u302TgTransgenic Insertion
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          sox32MO1-sox32MRPHLNO
          1 - 1 of 1
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          Fish
          No data available
          Antibodies
          Name Type Antigen Genes Isotypes Host Organism
          Ab1-ctnnbmonoclonal
            		IgG1Mouse
            1 - 1 of 1
            Show
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            RFPEFGRFP
            1 - 2 of 2
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            Mapping
            No data available
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            Citation
            ?apek, D., Smutny, M., Tichy, A.M., Morri, M., Janovjak, H., Heisenberg, C.P. (2019) Light-activated Frizzled7 reveals a permissive role of non-canonical Wnt signaling in mesendoderm cell migration. eLIFE. 8:.