PUBLICATION
In vitro and in vivo properties of the bovine antimicrobial peptide, Bactenecin 5
- Authors
- Price, R.L., Bugeon, L., Mostowy, S., Makendi, C., Wren, B.W., Williams, H.D., Willcocks, S.J.
- ID
- ZDB-PUB-190110-8
- Date
- 2019
- Source
- PLoS One 14: e0210508 (Journal)
- Registered Authors
- Bugeon, Laurence, Mostowy, Serge
- Keywords
- none
- MeSH Terms
-
- Bacillus/drug effects
- Cattle
- Macrophages/drug effects
- Macrophages/metabolism
- Zebrafish/embryology
- A549 Cells
- THP-1 Cells
- Neutrophils/drug effects
- Neutrophils/metabolism
- Amino Acid Sequence
- Humans
- Animals
- Transcription, Genetic/drug effects
- Chemokines/metabolism
- Antimicrobial Cationic Peptides/chemistry
- Antimicrobial Cationic Peptides/pharmacology*
- Mycobacterium marinum/drug effects
- Peptides, Cyclic/pharmacology*
- PubMed
- 30625198 Full text @ PLoS One
Citation
Price, R.L., Bugeon, L., Mostowy, S., Makendi, C., Wren, B.W., Williams, H.D., Willcocks, S.J. (2019) In vitro and in vivo properties of the bovine antimicrobial peptide, Bactenecin 5. PLoS One. 14:e0210508.
Abstract
Antimicrobial peptides (AMP), part of the innate immune system, are well studied for their ability to kill pathogenic microorganisms. However, many also possess important immunomodulatory effects, and this area has potential for the development of novel therapies to supplement traditional methods such as the use of antibiotics. Here, we characterise the microbicidal and immunomodulatory potential of the proline-rich bovine AMP, Bactenecin 5 (Bac5). We demonstrate broad antimicrobial activity, including against some mycobacterial species, which are important pathogens of fish, cattle and humans. Bac5 is able to activate macrophage-like THP-1 cells and can synergistically trigger the upregulation of tnf-α when co-stimulated with M. marinum. Furthermore, Bac5 sensitises A549 epithelial cells to stimulation with TNF-α. For the first time, we characterise the activity of Bac5 in vivo, and show it to be a potent chemokine for macrophages in the zebrafish (Danio rerio) embryo model of infection. Bac5 also supports the early recruitment of neutrophils in the presence of M. marinum. In the absence of host adaptive immunity, exogenous injected Bac5 is able to slow, although not prevent, infection of zebrafish with M. marinum.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping