PUBLICATION
Optical imaging of metabolic dynamics in animals
- Authors
- Shi, L., Zheng, C., Shen, Y., Chen, Z., Silveira, E.S., Zhang, L., Wei, M., Liu, C., de Sena-Tomas, C., Targoff, K., Min, W.
- ID
- ZDB-PUB-190108-19
- Date
- 2018
- Source
- Nature communications 9: 2995 (Journal)
- Registered Authors
- Targoff, Kimara
- Keywords
- none
- MeSH Terms
-
- Deuterium
- Zebrafish
- Macromolecular Substances
- Lipid Metabolism
- Lipids/chemistry
- Scattering, Radiation
- Caenorhabditis elegans
- Humans
- Animals
- Mice, Inbred C57BL
- Mice
- Neoplasm Transplantation
- Cell Line, Tumor
- Spectrum Analysis, Raman*
- Chlorocebus aethiops
- Deuterium Oxide
- Image Processing, Computer-Assisted
- COS Cells
- Carbon/chemistry
- HeLa Cells
- Optical Imaging/methods*
- Metabolism*
- Contrast Media
- PubMed
- 30082908 Full text @ Nat. Commun.
Citation
Shi, L., Zheng, C., Shen, Y., Chen, Z., Silveira, E.S., Zhang, L., Wei, M., Liu, C., de Sena-Tomas, C., Targoff, K., Min, W. (2018) Optical imaging of metabolic dynamics in animals. Nature communications. 9:2995.
Abstract
Direct visualization of metabolic dynamics in living animals with high spatial and temporal resolution is essential to understanding many biological processes. Here we introduce a platform that combines deuterium oxide (D2O) probing with stimulated Raman scattering (DO-SRS) microscopy to image in situ metabolic activities. Enzymatic incorporation of D2O-derived deuterium into macromolecules generates carbon-deuterium (C-D) bonds, which track biosynthesis in tissues and can be imaged by SRS in situ. Within the broad vibrational spectra of C-D bonds, we discover lipid-, protein-, and DNA-specific Raman shifts and develop spectral unmixing methods to obtain C-D signals with macromolecular selectivity. DO-SRS microscopy enables us to probe de novo lipogenesis in animals, image protein biosynthesis without tissue bias, and simultaneously visualize lipid and protein metabolism and reveal their different dynamics. DO-SRS microscopy, being noninvasive, universally applicable, and cost-effective, can be adapted to a broad range of biological systems to study development, tissue homeostasis, aging, and tumor heterogeneity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping