PUBLICATION
Plasma proteomic analysis of zebrafish following spring viremia of carp virus infection
- Authors
- Medina-Gali, R.M., Belló-Pérez, M., Ciordia, S., Mena, M.C., Coll, J., Novoa, B., Ortega-Villaizán, M.D.M., Perez, L.
- ID
- ZDB-PUB-181224-26
- Date
- 2018
- Source
- Fish & shellfish immunology 86: 892-899 (Journal)
- Registered Authors
- Novoa, Beatriz
- Keywords
- Plasma, Proteomics, SVCV, Vitellogenin, Zebrafish
- MeSH Terms
-
- Animals
- Fish Diseases/immunology
- Fish Proteins/immunology*
- Plasma/chemistry*
- Proteome/immunology*
- Rhabdoviridae/physiology
- Rhabdoviridae Infections/immunology
- Rhabdoviridae Infections/veterinary
- Zebrafish/blood
- Zebrafish/immunology*
- Zebrafish/metabolism
- PubMed
- 30580041 Full text @ Fish Shellfish Immunol.
Citation
Medina-Gali, R.M., Belló-Pérez, M., Ciordia, S., Mena, M.C., Coll, J., Novoa, B., Ortega-Villaizán, M.D.M., Perez, L. (2018) Plasma proteomic analysis of zebrafish following spring viremia of carp virus infection. Fish & shellfish immunology. 86:892-899.
Abstract
To better understand spring viremia of carp virus (SVCV) pathogenesis in zebrafish proteomic analysis was used to examine the plasma protein profile in SVCV-infected zebrafish. A total of 3062 proteins were identified. Of those 137, 63 and 31 proteins were enriched in blood samples harvested at 1, 2 and 5 days post SVCV infection, respectively. These altered host proteins were classified based on their biological function: 23 proteins under the response to stimulus term were identified. Interestingly, at the top of the up-regulated proteins during SVCV infection were the proteins of the vitellogenin family (Vtg) and the grass carp reovirus-induced gene (Gig) proteins. Real-time RT-PCR evaluation of samples from internal organs verified that SVCV infection induced vtg and gig2 gene expression already at day 1 post-infection. Western blot analysis revealed the presence of Vtg protein only in blood of SVCV-infected fish. This is the first proteomic study to reveal the involvement of Vtg proteins in adult fish response to viral challenge. It also highlights the role of Gig proteins as important factors in antiviral response in fish. This work provides valuable relevant insight into virus-host interaction and the identification of molecular markers of fish response to virus.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping