PUBLICATION
Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin
- Authors
- Pavic, A., Dragana, M.Ć., Nebojša, J., Biljana, N., Nataša, S., Branka, V., Jelena, K.V.
- ID
- ZDB-PUB-181216-1
- Date
- 2019
- Source
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 109: 2482-2491 (Journal)
- Registered Authors
- Keywords
- Allium extracts, Anti-angiogenesis, Anticancer activity, Cardio- and myeloprotection, Doxorubicin, Zebrafish embryos
- MeSH Terms
-
- A549 Cells
- Allium*
- Animals
- Antibiotics, Antineoplastic/toxicity*
- Dose-Response Relationship, Drug
- Doxorubicin/toxicity*
- Drug-Related Side Effects and Adverse Reactions/metabolism
- Drug-Related Side Effects and Adverse Reactions/pathology
- Drug-Related Side Effects and Adverse Reactions/prevention & control*
- Hep G2 Cells
- Humans
- Onions
- Plant Extracts/isolation & purification
- Plant Extracts/pharmacology*
- Plant Extracts/therapeutic use
- Zebrafish
- PubMed
- 30551509 Full text @ Biomed. Pharmacother.
Citation
Pavic, A., Dragana, M.Ć., Nebojša, J., Biljana, N., Nataša, S., Branka, V., Jelena, K.V. (2019) Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 109:2482-2491.
Abstract
The objective of this study was to evaluate potential of two chemically characterized edible wild onion species, Allium flavum and Allium carinatum, to reduce side effects of cytostatic doxorubicin (Dox). Since Dox application is mainly limited due to its high cardiotoxicity, while there are no approved cardioprotective agents for the prevention of Dox adverse effects, new co-treatments are urgently needed. Here, we showed that methanol extracts expressed high antioxidant activity and synergistically increased Dox anticancer activity against human hepatoma (HepG2) and lung carcinoma (A549) cells, while protected normal human fibroblasts (MRC-5) from Dox cytotoxicity. Analysis of the antioxidative enzymes level (catalase and superoxide dismutases) showed that the catalase level was differently altered in cancer cells compared to normal cells upon applied treatments. In vivo toxicity evaluation in the zebrafish model revealed significantly lower toxicity of extracts compared to Dox, and no teratogenic effects at applied doses. We found that extracts successfully rescued the Dox-treated embryos of life-threating cardiomyopathy, while at the same time reduced developmental toxicity and neutropenia. Further analysis demonstrated that extracts had higher anti-angiogenic activity than sunitinib or auranofin, clinically used antiangiogenic drugs. In addition, angiogenesis was markedly more suppressed in Dox-extract cotreatments than upon single treatments.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping