PUBLICATION
Toxicological evaluation of metal oxide nanoparticles and mixed exposures at low doses using zebra fish and THP1 cell line
- Authors
- Kaur, J., Khatri, M., Puri, S.
- ID
- ZDB-PUB-181215-20
- Date
- 2018
- Source
- Environmental toxicology 34(4): 375-387 (Journal)
- Registered Authors
- Keywords
- apoptosis, genotoxicity, nanotoxicology
- MeSH Terms
-
- Cell Survival/drug effects
- Dose-Response Relationship, Drug
- Apoptosis/drug effects
- Zebrafish*/embryology
- Water Pollutants, Chemical/chemistry
- Water Pollutants, Chemical/toxicity*
- Cell Culture Techniques
- Animals
- Cerium/chemistry
- Cerium/toxicity*
- Humans
- DNA Damage
- Cell Line
- Surface Properties
- Copper/chemistry
- Copper/toxicity*
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/pathology
- Nanoparticles/chemistry
- Nanoparticles/toxicity*
- PubMed
- 30548797 Full text @ Env. Tox.
Citation
Kaur, J., Khatri, M., Puri, S. (2018) Toxicological evaluation of metal oxide nanoparticles and mixed exposures at low doses using zebra fish and THP1 cell line. Environmental toxicology. 34(4):375-387.
Abstract
Metal and metal oxide nanoparticles are being used in different industries now-a-days leading to their unavoidable exposure to humans and animals. In the present study, toxicological testing was done using nanoparticles of copper oxide, cerium oxide and their mixture (1:1 ratio) on zebra fish embryos and THP-1 cell line. Zebrafish embryos were exposed to 0.01 μg/ml to 50 μg/ml concentrations of dispersed nanoparticles using a 96 well plate and their effects were studied at different hours post fertilization (hpf) i.e. 0 hpf, 24 hpf, 48 hpf, 72 hpf and 96 hpf respectively. Results showed that copper oxide nanoparticles has drastic effects on the morphology and physiology of zebra fish whereas cerium oxide nanoparticles and mixture of these nanoparticles did not show much of the effects. Comparable results were obtained from in vitro study using human monocyte cell line (THP-1). It is concluded that these nanoparticles may cause toxicological effects to humans and environment.
Genes / Markers
Figure Gallery (1 images)
Expression
Phenotype
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Human Disease / Model
Sequence Targeting Reagents
Fish
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