PUBLICATION
Voltage gating of mechanosensitive PIEZO channels
- Authors
- Moroni, M., Servin-Vences, M.R., Fleischer, R., Sánchez-Carranza, O., Lewin, G.R.
- ID
- ZDB-PUB-181213-9
- Date
- 2018
- Source
- Nature communications 9: 1096 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Line
- Drosophila Proteins/genetics
- Drosophila Proteins/metabolism*
- Drosophila melanogaster
- Evolution, Molecular
- Humans
- Ion Channels/genetics
- Ion Channels/metabolism*
- Mechanotransduction, Cellular*
- Mice
- Mutation, Missense
- Patch-Clamp Techniques
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 29545531 Full text @ Nat. Commun.
Citation
Moroni, M., Servin-Vences, M.R., Fleischer, R., Sánchez-Carranza, O., Lewin, G.R. (2018) Voltage gating of mechanosensitive PIEZO channels. Nature communications. 9:1096.
Abstract
Mechanosensitive PIEZO ion channels are evolutionarily conserved proteins whose presence is critical for normal physiology in multicellular organisms. Here we show that, in addition to mechanical stimuli, PIEZO channels are also powerfully modulated by voltage and can even switch to a purely voltage-gated mode. Mutations that cause human diseases, such as xerocytosis, profoundly shift voltage sensitivity of PIEZO1 channels toward the resting membrane potential and strongly promote voltage gating. Voltage modulation may be explained by the presence of an inactivation gate in the pore, the opening of which is promoted by outward permeation. Older invertebrate (fly) and vertebrate (fish) PIEZO proteins are also voltage sensitive, but voltage gating is a much more prominent feature of these older channels. We propose that the voltage sensitivity of PIEZO channels is a deep property co-opted to add a regulatory mechanism for PIEZO activation in widely different cellular contexts.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping