PUBLICATION

3‑Bromo‑5‑(ethoxymethyl)‑1,2‑benzenediol inhibits LPS-induced pro-inflammatory responses by preventing ROS production and downregulating NF-κB in vitro and in a zebrafish model

Authors
Ko, E.Y., Heo, S.J., Cho, S.H., Lee, W., Kim, S.Y., Yang, H.W., Ahn, G., Cha, S.H., Kwon, S.H., Jeong, M.S., Lee, K.P., Jeon, Y.J., Kim, K.N.
ID
ZDB-PUB-181212-26
Date
2018
Source
International Immunopharmacology   67: 98-105 (Journal)
Registered Authors
Jeon, You-Jin
Keywords
Anti-inflammatory, BEMB, NF-?B, RAW264.7 cells, ROS, Zebrafish embryo
MeSH Terms
  • Rhodophyta/immunology
  • Nitric Oxide Synthase Type II/genetics
  • Nitric Oxide Synthase Type II/metabolism
  • Disease Models, Animal
  • Anti-Inflammatory Agents/therapeutic use*
  • Fish Proteins/genetics
  • Fish Proteins/metabolism*
  • Mice
  • Lipopolysaccharides/immunology
  • Reactive Oxygen Species/metabolism
  • Inflammation/drug therapy*
  • Nitric Oxide/metabolism
  • Humans
  • Benzene/chemistry
  • Benzene/therapeutic use*
  • RAW 264.7 Cells
  • Zebrafish
  • Down-Regulation
  • Animals
  • Embryo, Nonmammalian
  • NF-kappa B/genetics
  • NF-kappa B/metabolism*
(all 22)
PubMed
30537636 Full text @ Int. Immunopharmacol.
Abstract
The anti-inflammatory effects of 3‑bromo‑5‑(ethoxymethyl)‑1,2‑benzenediol (BEMB) from Polysiphonia morrowii were evaluated in lipopolysaccharide (LPS)-induced RAW264.7 cells and zebrafish embryo. BEMB showed anti-inflammatory effects by inhibiting the production of nitric oxide (NO) and reactive oxygen species (ROS), and the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in the LPS-activated RAW264.7 cells and zebrafish embryo without cytotoxicity. Moreover, BEMB suppressed the protein and mRNA expression levels of nuclear factor (NF)-κB (p65 and inhibitor of NF-κB [IκB]-A) in RAW264.7 cells and zebrafish embryo, respectively. Collectively, the results of this study indicate that BEMB suppressed the production of pro-inflammatory mediators such as NO, iNOS, and COX-2 as well as their regulation in LPS-induced RAW264.7 cells and zebrafish embryos by inhibiting ROS production and NF-κB expression. Therefore, this study suggests that BEMB could be a potential anti-inflammatory agent for the treatment of inflammatory diseases.
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Citation
Ko, E.Y., Heo, S.J., Cho, S.H., Lee, W., Kim, S.Y., Yang, H.W., Ahn, G., Cha, S.H., Kwon, S.H., Jeong, M.S., Lee, K.P., Jeon, Y.J., Kim, K.N. (2018) 3‑Bromo‑5‑(ethoxymethyl)‑1,2‑benzenediol inhibits LPS-induced pro-inflammatory responses by preventing ROS production and downregulating NF-κB in vitro and in a zebrafish model. International Immunopharmacology. 67:98-105.