PUBLICATION
Naa15 knockdown enhances c2c12 myoblast fusion and induces defects in zebrafish myotome morphogenesis
- Authors
- Monestier, O., Landemaine, A., Bugeon, J., Rescan, P.Y., Gabillard, J.C.
- ID
- ZDB-PUB-181207-2
- Date
- 2018
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 228: 61-67 (Journal)
- Registered Authors
- Gabillard, Jean-Charles
- Keywords
- Morpholino, Muscle regeneration, siRNA screen
- MeSH Terms
-
- Animals
- Cell Fusion
- Gene Knockout Techniques
- Mice
- Muscle Development/physiology*
- Myoblasts/cytology
- Myoblasts/metabolism*
- N-Terminal Acetyltransferase A/genetics
- N-Terminal Acetyltransferase A/metabolism*
- N-Terminal Acetyltransferase E/genetics
- N-Terminal Acetyltransferase E/metabolism*
- Zebrafish/embryology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 30502388 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Monestier, O., Landemaine, A., Bugeon, J., Rescan, P.Y., Gabillard, J.C. (2018) Naa15 knockdown enhances c2c12 myoblast fusion and induces defects in zebrafish myotome morphogenesis. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 228:61-67.
Abstract
The understanding of muscle tissue formation and regeneration is essential for the development of therapeutic approaches to treat muscle diseases or loss of muscle mass and strength during ageing or cancer. One of the critical steps in muscle formation is the fusion of muscle cells to form or regenerate muscle fibres. To identify new genes controlling myoblast fusion, we performed a siRNA screen in c2c12 myoblasts. The genes identified during this screen were then studied in vivo by knockdown in zebrafish using morpholino. We found that N-alpha-acetyltransferase 15 (Naa15) knockdown enhanced c2c12 myoblast fusion, suggesting that Naa15 negatively regulates myogenic cell fusion. We identified two Naa15 orthologous genes in the zebrafish genome: Naa15a and Naa15b. These two orthologs were expressed in the myogenic domain of the somite. Knockdown of zebrafish Naa15a and Naa15b genes induced a "U"-shaped segmentation of the myotome and alteration of myotome boundaries, resulting in the formation of abnormally long myofibres spanning adjacent somites. Taken together, these results show that Naa15 regulates myotome formation and myogenesis in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping