PUBLICATION
Zebrafish embryos exposed to deltamethrin exhibited abnormalities in spite of induced expression of related genes ( you, you-too, momo and u-boot)
- Authors
- Shabnam, K.R., Gangappa, D., Philip, G.H.
- ID
- ZDB-PUB-181207-19
- Date
- 2018
- Source
- Toxicology and industrial health 35(1): 11-19 (Journal)
- Registered Authors
- Keywords
- Deltamethrin, momo, u-boot, yot-too, you, zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/abnormalities
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Extracellular Matrix Proteins/genetics
- Extracellular Matrix Proteins/metabolism
- Genes, Developmental/drug effects
- Genes, Developmental/genetics
- Insecticides/toxicity*
- Nitriles/toxicity*
- Notochord/drug effects
- Notochord/embryology
- Pyrethrins/toxicity*
- Somites/drug effects
- Somites/embryology
- Zebrafish/abnormalities*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zinc Finger Protein Gli2/genetics
- Zinc Finger Protein Gli2/metabolism
- PubMed
- 30518298 Full text @ Toxicol. Ind. Health
Citation
Shabnam, K.R., Gangappa, D., Philip, G.H. (2018) Zebrafish embryos exposed to deltamethrin exhibited abnormalities in spite of induced expression of related genes ( you, you-too, momo and u-boot). Toxicology and industrial health. 35(1):11-19.
Abstract
Evaluation of the toxic effects of a widely used synthetic pyrethroid, deltamethrin (DM), was carried out in this study. This pesticide is preferred for pest control because of its low environmental persistence and toxicity. We investigated the expression pattern of four genes, namely, you ( you), yot ( you-too), momo ( mom) and ubo ( u-boot) during early development of zebrafish, that is, from 12 hpf to 48 hpf stages. These stages are selected as most of the important developmental aspects take place during this period. All four genes are known to play a vital role in development of notochord and somites. To understand the effect of DM on development, embryos of 4 hpf stage were exposed to two concentrations (100 and 200 µg/L) of DM, and observations were made at 12, 24 and 48 hpf stages. Our earlier studies have shown phenotypic abnormalities such as notochord bending, tail deformation, yolk sac and pericardial edema, lightening of body and eye pigmentation and interfered in somite patterning, during these stages of development. Understanding the relationship of phenotypic abnormalities with these four genes has been our primary objective. These four genes were analyzed by Reverse transcription (RT)-polymerase chain reaction and intensity of the bands has shown induction in their expression after exposure to the toxicant. In spite of the expression of genes, it was noticed that DM caused abnormalities. It can be said from the results that translational pathway could have been affected.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping