PUBLICATION
The toxicity of 2,6-dichlorobenzoquinone on the early life stage of zebrafish: A survey on the endpoints at developmental toxicity, oxidative stress, genotoxicity and cytotoxicity
- Authors
- Sun, H.J., Zhang, Y., Zhang, J.Y., Lin, H., Chen, J., Hong, H.
- ID
- ZDB-PUB-181204-4
- Date
- 2018
- Source
- Environmental pollution (Barking, Essex : 1987) 245: 719-724 (Journal)
- Registered Authors
- Keywords
- Cytotoxicity, Dichlorobenzoquinone, Genotoxicity, Lipid peroxidation, Oxidative stress, Zebrafish
- MeSH Terms
-
- Animals
- Apoptosis/drug effects
- Benzoquinones/toxicity*
- DNA Damage
- Embryo, Nonmammalian/drug effects*
- Lipid Peroxidation/drug effects
- Malondialdehyde/metabolism
- Oxidative Stress/drug effects
- Water Pollutants, Chemical/toxicity*
- Zebrafish/genetics
- Zebrafish/growth & development*
- PubMed
- 30500751 Full text @ Environ. Pollut.
Citation
Sun, H.J., Zhang, Y., Zhang, J.Y., Lin, H., Chen, J., Hong, H. (2018) The toxicity of 2,6-dichlorobenzoquinone on the early life stage of zebrafish: A survey on the endpoints at developmental toxicity, oxidative stress, genotoxicity and cytotoxicity. Environmental pollution (Barking, Essex : 1987). 245:719-724.
Abstract
2,6-dichlorobenzoquinone (2,6-DCBQ), an emerging disinfection by-production, frequently occurs in reclaimed water and drinking water. However, limited information was available regarding its toxicity. To evaluate its impact, zebrafish at early life stage were exposed to 0, 10, 30, 60, 90, or 120 μg L-1 2,6-BDCQ for 72 h. Our results indicated that 2,6-BDCQ decreased zebrafish's survival rate to 65% and 44% at 90 and 120 μg L-1 treatments and increased its aberration rate to 11% and 26% at 90 μg L-1 and 120 μg L-1 treatments. Besides, 2,6-BDCQ had adverse effect on its oxidative stress (elevated superoxide dismutase activity), lipid peroxidation (increased malondialdehyde levels), DNA damage (increased 8-hydroxydeoxyguanosine contents) and apoptosis (increased caspase-3 activity). Although lower concentrations (≤60 μg L-1) of 2,6-BDCQ didn't exhibit significant effect on its survival development or lipid peroxidation of zebrafish, they induced obvious DNA damage and apoptosis occurrence. These results revealed 2,6-BDCQ caused genotoxicity and cytotoxicity to zebrafish. This study provides novel insight into 2,6-DCBQ-induced toxicity in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping