|ZFIN ID: ZDB-PUB-181130-18|
Dynamic and non-contact 3D sample rotation for microscopy
Berndt, F., Shah, G., Power, R.M., Brugués, J., Huisken, J.
|Source:||Nature communications 9: 5025 (Journal)|
|Registered Authors:||Huisken, Jan|
|PubMed:||30487638 Full text @ Nat. Commun.|
Berndt, F., Shah, G., Power, R.M., Brugués, J., Huisken, J. (2018) Dynamic and non-contact 3D sample rotation for microscopy. Nature communications. 9:5025.
ABSTRACTPrecise sample orientation is crucial for microscopy but is often performed with macroscopic tools and low accuracy. In vivo imaging of growing and developing samples even requires dynamic adaptation of the sample orientation to continuously achieve optimal imaging. Here, we present a method for freely positioning a sample in 3D by introducing magnetic beads and applying a magnetic field. We demonstrate magnetic orientation of fixed mouse embryos and artemia, and live zebrafish embryos and larvae on an epi-fluorescence microscope and on a light-sheet system for optimal imaging.