PUBLICATION
Exposure to DBP induces the toxicity in early development and adverse effects on cardiac development in zebrafish (Danio rerio)
- Authors
- Sun, G., Li, Y.
- ID
- ZDB-PUB-181127-63
- Date
- 2018
- Source
- Chemosphere 218: 76-82 (Journal)
- Registered Authors
- Keywords
- Cardiac development, DBP, Developmental toxicity, Zebrafish embryos
- MeSH Terms
-
- Abnormalities, Multiple/chemically induced
- Animals
- Dibutyl Phthalate/toxicity*
- Embryo, Nonmammalian/drug effects
- Embryonic Development/drug effects
- Heart/drug effects
- Heart/growth & development
- Plasticizers/toxicity
- Transcription Factors/drug effects
- Transcription Factors/metabolism
- Zebrafish/embryology
- Zebrafish/growth & development*
- Zebrafish Proteins/drug effects*
- Zebrafish Proteins/metabolism
- PubMed
- 30469006 Full text @ Chemosphere
Citation
Sun, G., Li, Y. (2018) Exposure to DBP induces the toxicity in early development and adverse effects on cardiac development in zebrafish (Danio rerio). Chemosphere. 218:76-82.
Abstract
Dibutyl phthalate (DBP) is one of the most ubiquitous plasticizers used worldwide and has been frequently detected in soil, water, atmosphere, and other environmental media. DBP has become a ubiquitous environment contaminant and causes serious pollution. However, much attention has been paid to the toxicity of DBP, with only limited attention paid to its detrimental effects on the heart. In the present study, we investigated the toxicity of DBP in zebrafish embryo development, especially adverse effects on cardiac development. Embryos at 4-h post-fertilization (hpf) were exposed to different concentrations of DBP (0, 0.36, 1.8 and 3.6 μM) until 72 hpf. Exposure to DBP resulted in morphological abnormalities in zebrafish embryos. Exposure to 1.8 μM DBP significantly affected the growth, malformation rate, cardiac malformation rate and cardiac looping. Exposure to 3.6 μM DBP significantly affected all endpoints. To preliminarily understand the underlying mechanisms of toxic effects of DBP on the embryo heart, we examined the expression of master cardiac transcription factors such as NKX2.5 and TBX5. The expression of this two transcription factors was significantly reduced with DBP treatment in a dose-dependent manner. Our results demonstrate that exposure to DBP resulted in zebrafish developmental toxicity, pericardial edema, cardiac structure deformities and function alteration, and changed the expression of master cardiac transcription factors such as NKX2.5 and TBX5.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping