PUBLICATION
TiO2 nanoparticles and BPA are combined to impair the development of offspring zebrafish after parental coexposure
- Authors
- Chen, L., Hu, C., Guo, Y., Shi, Q., Zhou, B.
- ID
- ZDB-PUB-181119-8
- Date
- 2018
- Source
- Chemosphere 217: 732-741 (Journal)
- Registered Authors
- Guo, YongYong, Zhou, BingSheng
- Keywords
- Developmental toxicity, Parental combined exposure, Phagolysosome, Sex hormones, TiO(2) and BPA mixture
- MeSH Terms
-
- Animals
- Benzhydryl Compounds/toxicity*
- Estradiol/analysis
- Female
- Larva/chemistry
- Male
- Maternal Exposure/adverse effects*
- Nanoparticles/chemistry
- Nanoparticles/toxicity
- Paternal Exposure/adverse effects*
- Phenols/toxicity*
- Testosterone/analysis
- Titanium/toxicity*
- Vitellogenins
- Water Pollutants, Chemical/toxicity
- Zebrafish/embryology
- Zebrafish/growth & development*
- PubMed
- 30448753 Full text @ Chemosphere
Citation
Chen, L., Hu, C., Guo, Y., Shi, Q., Zhou, B. (2018) TiO2 nanoparticles and BPA are combined to impair the development of offspring zebrafish after parental coexposure. Chemosphere. 217:732-741.
Abstract
Titanium dioxide (TiO2) nanoparticles and bisphenol A (BPA) in aquatic environments interact reciprocally to enhance the maternal transfer of pollutants to offspring, thus varying the innate toxicities during early embryonic development. However, it remains unexplored regarding the molecular mechanisms of developmental toxicity in offspring after parental coexposure. In the present study, adult zebrafish were exposed to TiO2 nanoparticles (100 μg/L), BPA (20 μg/L) or their binary mixture for four months. Then, eggs of F1 generation were collected and reared in clean water until 5 days post-fertilization. In characteristic of larval survival and growth, parental coexposure to TiO2 particles and BPA caused a severer inhibition of F1 offspring larvae compared with single exposure. Mechanistic investigation by shotgun proteomics found that development of larval offspring from coexposed parents was impaired through a distinct mode of toxicity, that is, specifically altering the activity of phagosome and lysosome. Single exposure of adult zebrafish to TiO2 mainly affected insulin-responsive compartment; and BPA parental exposure mainly affected carbohydrate metabolism and calcium signaling of larval offspring. Furthermore, considering the tight regulation of sex hormones in the expression of vitellogenin (VTG), addition of nanoparticles during parental exposure led to inconsistencies between VTG induction and endogenous levels of sex hormones (estradiol and testosterone) in F1 offspring fish. This implied that transfer of nanoparticles to offspring larvae may change the availability of hormonal molecules and BPA at target tissues. Overall, current results provided mechanistic clues into the multigenerational developmental toxicity by parental coexposure to TiO2 particles and BPA.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping