PUBLICATION
mRNA expression of antioxidant and biotransformation enzymes in zebrafish (Danio rerio) embryos after exposure to the tricyclic antidepressant amitriptyline
- Authors
- Sehonova, P., Zikova, A., Blahova, J., Svobodova, Z., Chloupek, P., Kloas, W.
- ID
- ZDB-PUB-181118-10
- Date
- 2018
- Source
- Chemosphere 217: 516-521 (Journal)
- Registered Authors
- Keywords
- CAT, CYP1A, Fish, GPx, GST, SOD
- MeSH Terms
-
- Amitriptyline/pharmacology
- Amitriptyline/therapeutic use*
- Animals
- Embryonic Development/drug effects*
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- RNA, Messenger/metabolism*
- Antidepressive Agents, Tricyclic/pharmacology
- Antidepressive Agents, Tricyclic/therapeutic use*
- Zebrafish
- Antioxidants/metabolism*
- Oxidative Stress/drug effects
- PubMed
- 30445396 Full text @ Chemosphere
Citation
Sehonova, P., Zikova, A., Blahova, J., Svobodova, Z., Chloupek, P., Kloas, W. (2018) mRNA expression of antioxidant and biotransformation enzymes in zebrafish (Danio rerio) embryos after exposure to the tricyclic antidepressant amitriptyline. Chemosphere. 217:516-521.
Abstract
Now-a-days, the occurrence of antidepressant residues in surface waters has become a major concern. Amitriptyline (AMI) has been described to treat depression and other disorders for decades. However, little is known about its effect on non-target organisms. The aim of this study was to assess the potential impact of AMI on the mRNA expression of antioxidant and detoxification enzymes during the early embryonic development of zebrafish (Danio rerio). Fertilized D. rerio embryos were exposed to AMI at concentrations of 300 ng/L and 30 μg/L and sampled 24, 48, 96, and 144 h post fertilization (hpf) to assess the mRNA expressions of cytochrome P450 1A1, glutathione-S-transferase, glutathione peroxidase, superoxide dismutase, and catalase. The time courses of the mRNA expressions of antioxidant and detoxification enzymes revealed characteristic changes during embryonic development causing generally transient changes post hatching; however, AMI did not cause any significant impact except in the case of CAT after 144 h, which was significantly upregulated by the AMI concentration of 30 μg/L. The results suggest that the antidepressant AMI causes only moderate to minor impacts on antioxidant and detoxification enzymes during early embryonic development of the non-target organism D. rerio and that CAT is the only biomarker affected by AMI.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping