PUBLICATION
Slice-illuminated optical projection tomography
- Authors
- Davis, S.P.X., Wisniewski, L., Kumar, S., Correia, T., Arridge, S.R., Frankel, P., McGinty, J., French, P.M.W.
- ID
- ZDB-PUB-181116-9
- Date
- 2018
- Source
- Optics letters 43: 5555-5558 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 30439894 Full text @ Opt. Lett.
Citation
Davis, S.P.X., Wisniewski, L., Kumar, S., Correia, T., Arridge, S.R., Frankel, P., McGinty, J., French, P.M.W. (2018) Slice-illuminated optical projection tomography. Optics letters. 43:5555-5558.
Abstract
To improve the imaging performance of optical projection tomography (OPT) in live samples, we have explored a parallelized implementation of semi-confocal line illumination and detection to discriminate against scattered photons. Slice-illuminated OPT (sl-OPT) improves reconstruction quality in scattering samples by reducing interpixel crosstalk at the cost of increased acquisition time. For in vivo imaging, this can be ameliorated through the use of compressed sensing on angularly undersampled OPT data sets. Here, we demonstrate sl-OPT applied to 3D imaging of bead phantoms and live adult zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping