PUBLICATION
Characterization and expression of melanin-concentrating hormone (MCH) in common carp (Cyprinus carpio) during fasting and reproductive cycle
- Authors
- Xu, J., Hou, F., Wang, D., Li, J., Yang, G.
- ID
- ZDB-PUB-181115-5
- Date
- 2018
- Source
- Fish physiology and biochemistry 45(2): 805-817 (Journal)
- Registered Authors
- Keywords
- Cloning, Cyprinus carpio, Fasting, MCH, Reproduction, Tissue distribution
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Carps/metabolism*
- Cloning, Molecular
- Food Deprivation*
- Gene Expression Regulation
- Hypothalamic Hormones/genetics
- Hypothalamic Hormones/metabolism*
- Hypothalamus
- Melanins/genetics
- Melanins/metabolism*
- Pituitary Hormones/genetics
- Pituitary Hormones/metabolism*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Reproduction/physiology*
- Transcriptome
- PubMed
- 30426273 Full text @ Fish Physiol. Biochem.
Citation
Xu, J., Hou, F., Wang, D., Li, J., Yang, G. (2018) Characterization and expression of melanin-concentrating hormone (MCH) in common carp (Cyprinus carpio) during fasting and reproductive cycle. Fish physiology and biochemistry. 45(2):805-817.
Abstract
Melanin-concentrating hormone (MCH) was initially known as a regulator of teleost skin color and possesses multiple functions in mammals, such as the regulation of energy balance and reproduction. However, the role of MCH in fish remains unclear. In the present study, a 590 bp cDNA fragment of common carp (Cyprinus carpio) MCH gene was cloned. Amino acid sequence similarities with other teleost ranged from 23 to 93%. The mature MCH peptide (DTMRCMVGRVYRPCWEV) located in the C-terminal region of MCH precursor was 100% identical to that of goldfish, zebrafish, chum salmon, and rainbow trout. Tissue expression profiles showed that MCH mRNA was ubiquitously expressed throughout the brain and peripheral tissues and highly expressed in the brain and pituitary. Within the brain, MCH mRNA was expressed preponderantly in the hypothalamus. MCH mRNA expression in the hypothalamus was increased after feeding, decreased after 3, 5, or 7 days fasting, and increased upon refeeding. These results suggested that MCH might have anorexigenic actions in common carp. Meanwhile, MCH gene expression varied based on reproductive cycle, which might be related to the long-term regulation of MCH in energy balance. In conclusion, our novel finding revealed that MCH was involved in the regulation of appetite and energy balance in common carp.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping