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ZFIN ID: ZDB-PUB-181114-10
Zebrafish fatty acids receptor Gpr84 enhances macrophage phagocytosis
Wang, M., Zhang, X., Zhang, S., Liu, Z.
Date: 2018
Source: Fish & shellfish immunology   84: 1098-1099 (Other)
Registered Authors: Liu, Zhenhui
Keywords: Fatty acids receptor, Gpr84, Phagocytosis, Zebrafish
MeSH Terms:
  • Animals
  • Escherichia coli/physiology
  • Flow Cytometry
  • Gene Expression
  • Macrophages/physiology*
  • Phagocytosis/genetics*
  • Receptors, G-Protein-Coupled/genetics*
  • Receptors, G-Protein-Coupled/immunology*
  • Staphylococcus aureus/physiology
  • Zebrafish/genetics
  • Zebrafish/immunology
  • Zebrafish/physiology*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/immunology*
PubMed: 30414894 Full text @ Fish Shellfish Immunol.
GPR84 was identified as a receptor for medium-chain fatty acids with carbon chain lengths of 9-14. It has previously been reported that lipopolysaccharide (LPS) induces significantly up-regulation of zebrafish gpr84, and zebrafish gpr84 overexpression markedly increased the LPS-stimulated production of the cytokine IL-12. Here we expanded on these studies to further investigate the roles of zebrafish Gpr84 in immune reaction. Flow cytometric assay was used to assess the effects of zebrafish Gpr84 on the phagocytosis of bacteria by macrophages. It was found that overexpression of zebrafish gpr84 significantly increased both the phagocytic ability (PA) and phagocytic index (PI) values of the macrophages engulfing the bacteria, suggesting that zebrafish Gpr84 was able to promote the phagocytosis of bacteria by the macrophages. The data proves the direct effect of Gpr84 in immune reaction.