PUBLICATION

Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns

Authors
Yamakawa, N., Vanbeselaere, J., Chang, L.Y., Yu, S.Y., Ducrocq, L., Harduin-Lepers, A., Kurata, J., Aoki-Kinoshita, K.F., Sato, C., Khoo, K.H., Kitajima, K., Guerardel, Y.
ID
ZDB-PUB-181109-3
Date
2018
Source
Nature communications   9: 4647 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Aging/metabolism*
  • Animals
  • Brain/metabolism
  • Gene Expression Regulation
  • Glycomics/methods*
  • Glycosphingolipids/isolation & purification
  • Glycosylation
  • Intestines
  • N-Acetylneuraminic Acid/metabolism*
  • Organ Specificity*
  • Polysaccharides/isolation & purification
  • Systems Biology/methods*
  • Zebrafish/metabolism*
PubMed
30405127 Full text @ Nat. Commun.
Abstract
The emergence of zebrafish Danio rerio as a versatile model organism provides the unique opportunity to monitor the functions of glycosylation throughout vertebrate embryogenesis, providing insights into human diseases caused by glycosylation defects. Using a combination of chemical modifications, enzymatic digestion and mass spectrometry analyses, we establish here the precise glycomic profiles of eight individual zebrafish organs and demonstrate that the protein glycosylation and glycosphingolipid expression patterns exhibits exquisite specificity. Concomitant expression screening of a wide array of enzymes involved in the synthesis and transfer of sialic acids shows that the presence of organ-specific sialylation motifs correlates with the localized activity of the corresponding glycan biosynthesis pathways. These findings provide a basis for the rational design of zebrafish lines expressing desired glycosylation profiles.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes