PUBLICATION

Copper inhibits hatching of fish embryos via inducing reactive oxygen species and down-regulating Wnt signaling

Authors
Zhang, Y., Zhang, R., Sun, H., Chen, Q., Yu, X., Zhang, T., Yi, M., Liu, J.X.
ID
ZDB-PUB-181103-12
Date
2018
Source
Aquatic toxicology (Amsterdam, Netherlands)   205: 156-164 (Journal)
Registered Authors
Liu, Jing-xia, Zhang, Ting
Keywords
Cu(2+), CuNPs, Hatching, ROS, Wnt signaling
MeSH Terms
  • Animals
  • Copper/toxicity*
  • Down-Regulation/drug effects*
  • Embryo, Nonmammalian/drug effects*
  • Reactive Oxygen Species/metabolism*
  • Water Pollutants, Chemical/toxicity
  • Wnt Signaling Pathway/genetics*
  • Zebrafish/embryology
  • Zebrafish/physiology*
PubMed
30388615 Full text @ Aquat. Toxicol.
Abstract
The copper ion (Cu2+) has been reported to suppress the hatching of fish. However, little is known about the underlying mechanism. In this study, copper nanoparticles (CuNPs) and Cu2+ were shown to significantly suppress hatching of zebrafish in a dosage-dependent manner, and reactive oxygen species (ROS) scavengers NAC (N-acetylcysteine) & GSH (reduced glutathione) and Wnt signaling agonist BIO (6-bromoindirubin-3'-oxime) significantly alleviated the suppressing effects of Cu2+ and CuNPs on egg hatching. Mechanistically, NAC, GSH, and BIO recovered the egg hatching in copper-treated group via increasing the embryonic motility rather than stimulating the expression and secretion of hatching enzymes before hatching. Additionally, no significant difference in egg hatching was observed between the control and Cu2+-treated group at 72 hpf (hours post fertilization) in cox17 mutant embryos, in which little ROS was producd after copper stimulation. This may be the first report that Cu2+ and CuNPs suppress embryonic motility and the subsequent hatching via inducing ROS and at the same time down-regulating Wnt signaling in fish embryos.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping