PUBLICATION

Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury

Authors
Ratnayake, D., Currie, P.D.
ID
ZDB-PUB-181028-6
Date
2019
Source
Methods in molecular biology (Clifton, N.J.)   1889: 245-254 (Other)
Registered Authors
Currie, Peter D., Ratnayake, Danni
Keywords
FACS, Muscle injury, Muscle progenitors, Muscle stem cells, Needle stab injury, Regeneration, Satellite cells, Skeletal muscle, Zebrafish larvae
MeSH Terms
  • Animals
  • Biomarkers*
  • Flow Cytometry*/methods
  • Immunophenotyping
  • Muscles/injuries*
  • Myoblasts/cytology*
  • Myoblasts/metabolism*
  • Phenotype
  • Regeneration
  • Zebrafish
PubMed
30367418 Full text @ Meth. Mol. Biol.
Abstract
This chapter describes a protocol for the isolation of larval zebrafish muscle stem/progenitor cells by fluorescence-activated cell sorting (FACS). This method has been successfully applied to isolate pax3a expressing cells 3 days following needle stab skeletal muscle injury. The cell sorting strategy described here can easily be adapted to any cell type at embryonic or larval stages. RNA extracted from the sorted cells can be used for subsequent downstream applications such as quantitative PCR (qPCR), microarrays, or next generation sequencing.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping