PUBLICATION

An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae

Authors
Bresciani, E., Broadbridge, E., Liu, P.P.
ID
ZDB-PUB-181027-10
Date
2018
Source
MethodsX   5: 1287-1290 (Other)
Registered Authors
Bresciani, Erica, Liu, Pu Paul
Keywords
Dissociation, Larvae dissociation to single cell suspension, Single cell suspension, Zebrafish, Zebrafish embryo
MeSH Terms
none
PubMed
30364607 Full text @ MethodsX
Abstract
Zebrafish (Danio rerio) has emerged as a powerful animal model to study developmental processes and human diseases. The introduction of CRISPR/Cas9 as a genome editing tool allowed the generation of genetic mutants with high-throughput (Varshney et al., 2015) and has opened the possibility to understand gene function not only during embryonic stages but also in larval stages. Therefore, there is an increasing need to optimize methods for embryo and larvae dissociation that allow the generation of single cell suspension for fluorescence-activated cell sorting (FACS), RNA extraction and single cell RNA-sequencing.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes