PUBLICATION
NADPH-Oxidase-derived reactive oxygen species are required for cytoskeletal organization, proper localization of E-cadherin and cell motility during zebrafish epiboly
- Authors
- Mendieta-Serrano, M.A., Mendez-Cruz, F.J., Antúnez-Mojica, M., Schnabel, D., Alvarez, L., Cárdenas, L., Lomelí, H., Ruiz-Santiesteban, J.A., Salas-Vidal, E.
- ID
- ZDB-PUB-181021-3
- Date
- 2018
- Source
- Free radical biology & medicine 130: 82-98 (Journal)
- Registered Authors
- Lomeli, Hilda, Salas-Vidal, Enrique, Schnabel, Denhi
- Keywords
- E-cadherin, NADPH oxidases, Reactive oxygen species, VAS2870, cell adhesion, cell motility, cytoskeleton, epiboly, extracellular signaling, hydrogen peroxide, zebrafish
- MeSH Terms
-
- Animals
- Blastoderm/metabolism*
- Cadherins/metabolism
- Cell Adhesion
- Cell Movement
- Cytoskeleton/metabolism*
- Embryo, Nonmammalian
- Morphogenesis
- NADPH Oxidases/metabolism*
- Reactive Oxygen Species/metabolism*
- Zebrafish/physiology*
- Zebrafish Proteins/metabolism
- PubMed
- 30342187 Full text @ Free Radic. Biol. Med.
Citation
Mendieta-Serrano, M.A., Mendez-Cruz, F.J., Antúnez-Mojica, M., Schnabel, D., Alvarez, L., Cárdenas, L., Lomelí, H., Ruiz-Santiesteban, J.A., Salas-Vidal, E. (2018) NADPH-Oxidase-derived reactive oxygen species are required for cytoskeletal organization, proper localization of E-cadherin and cell motility during zebrafish epiboly. Free radical biology & medicine. 130:82-98.
Abstract
Cell movements are essential for morphogenesis during animal development. Epiboly is the first morphogenetic process in zebrafish in which cells move en masse to thin and spread the deep and enveloping cell layers of the blastoderm over the yolk cell. While epiboly has been shown to be controlled by complex molecular networks, the contribution of reactive oxygen species (ROS) to this process has not previously been studied. Here, we show that ROS are required for epiboly in zebrafish. Visualization of ROS in whole embryos revealed dynamic patterns during epiboly progression. Significantly, inhibition of NADPH oxidase activity leads to a decrease in ROS formation, delays epiboly, alters E-cadherin and cytoskeleton patterns and, by 24hours post-fertilization, decreases embryo survival, effects that are rescued by hydrogen peroxide treatment. Our findings suggest that a delicate ROS balance is required during early development and that disruption of that balance interferes with cell adhesion, leading to defective cell motility and epiboly progression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping