PUBLICATION
Adriamycin inhibits embryonic development in zebrafish through downregulation of Kruppel-like factor4
- Authors
- Akthar, I.S.T., Pichiah, P.B.T., Arunachalam, S., Raja, S.
- ID
- ZDB-PUB-181005-9
- Date
- 2019
- Source
- Journal of biochemical and molecular toxicology : e22235 (Journal)
- Registered Authors
- Keywords
- Kruppel-like factor4 (Klf4), adriamycin, embryogenesis, hatching
- MeSH Terms
- none
- PubMed
- 30286259 Full text @ J. Biochem. Mol. Toxicol.
- CTD
- 30286259
Citation
Akthar, I.S.T., Pichiah, P.B.T., Arunachalam, S., Raja, S. (2019) Adriamycin inhibits embryonic development in zebrafish through downregulation of Kruppel-like factor4. Journal of biochemical and molecular toxicology. :e22235.
Abstract
Adriamycin is an effective anticancer drug used in a wide range of cancers. Anticancer drugs modulate oncogenes and nodal regulatory molecules that affect cell differentiation and organismal development. In this study, we explore the effect of adriamycin on Kruppel-like factor4 (Klf4), an essential pluripotent factor by choosing zebrafish embryos as a model system. Klf4 is involved in the regulation of cellular growth, proliferation, and differentiation. In zebrafish embryogenesis, Klf4 is a major regulator of differentiation of polster in the anterior mesendoderm region of cells into hatching gland cells. The importance of this study is to check the effect of adriamycin on embryonic development. We found, adriamycin dose dependently altered the gene expression level of Klf4 that occurs in parallel to its detrimental effect on hatching. Supportively, cathepsin L and cyclase-associated protein1 are the other two markers of hatching that are altered along with Klf4.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping