PUBLICATION

Deadenylation by the CCR4-NOT complex contributes to the turnover of hairy-related mRNAs in the zebrafish segmentation clock

Authors
Fujino, Y., Yamada, K., Sugaya, C., Ooka, Y., Ovara, H., Ban, H., Akama, K., Otosaka, S., Kinoshita, H., Yamasu, K., Mishima, Y., Kawamura, A.
ID
ZDB-PUB-181004-8
Date
2018
Source
FEBS letters   592(20): 3388-3398 (Journal)
Registered Authors
Kawamura, Akinori, Mishima, Yuichiro, Yamasu, Kyo
Keywords
3?UTR, Somite segmentation, Zebrafish, deadenylation, decapping, mRNA decay
MeSH Terms
  • RNA, Messenger/genetics*
  • RNA, Messenger/metabolism
  • Basic Helix-Loop-Helix Transcription Factors/genetics*
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Exoribonucleases/genetics
  • Exoribonucleases/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Gene Expression Regulation, Developmental*
  • Animals
  • Somites/embryology
  • Somites/metabolism*
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • RNA Processing, Post-Transcriptional
  • Body Patterning/genetics
  • Mesoderm/embryology
  • Mesoderm/metabolism
  • Animals, Genetically Modified
  • Biological Clocks
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
(all 23)
PubMed
30281784 Full text @ FEBS Lett.
Abstract
In the zebrafish segmentation clock, the hairy/enhancer of split-related genes her1, her7, and hes6 encode components of core oscillators. Since the expression of cyclic genes proceeds rapidly in the presomitic mesoderm (PSM), these hairy-related mRNAs are subject to strict post-transcriptional regulation. In this study, we demonstrate that inhibition of the CCR4-NOT deadenylase complex lengthens poly(A) tails of hairy-related mRNAs and increases the amount of these mRNAs, which is accompanied by defective somite segmentation. In transgenic embryos, we show that EGFP mRNAs with 3'UTRs of hairy-related genes exhibit turnover similar to endogenous mRNAs. Our results suggest that turnover rates of her1, her7, and hes6 mRNAs are differently regulated by the CCR4-NOT deadenylase complex possibly through their 3'UTRs in the zebrafish PSM. This article is protected by copyright. All rights reserved.
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Citation
Fujino, Y., Yamada, K., Sugaya, C., Ooka, Y., Ovara, H., Ban, H., Akama, K., Otosaka, S., Kinoshita, H., Yamasu, K., Mishima, Y., Kawamura, A. (2018) Deadenylation by the CCR4-NOT complex contributes to the turnover of hairy-related mRNAs in the zebrafish segmentation clock. FEBS letters. 592(20):3388-3398.