PUBLICATION
Transcriptome analysis reveals functional roles of nacreous protein N16 in prednisolone-induced osteoporotic zebrafish
- Authors
- Lin, J.B., Wu, H., Liu, Y.L., Shaw, P.C., Li, P.B.
- ID
- ZDB-PUB-180917-3
- Date
- 2018
- Source
- International journal of biological macromolecules 122: 1071-1079 (Journal)
- Registered Authors
- Keywords
- N16, Osteoporosis, Transcriptomic sequencing, Zebrafish
- MeSH Terms
-
- Animals
- Bone Density/drug effects
- Gene Expression Profiling*
- Molecular Sequence Annotation
- Osteoclasts/drug effects
- Osteoclasts/metabolism
- Osteoporosis/chemically induced
- Osteoporosis/drug therapy*
- Osteoporosis/genetics*
- Osteoporosis/pathology
- Prednisolone/adverse effects*
- Proteins/pharmacology*
- Proteins/therapeutic use
- Zebrafish*
- PubMed
- 30219513 Full text @ Int. J. Biol. Macromol.
Citation
Lin, J.B., Wu, H., Liu, Y.L., Shaw, P.C., Li, P.B. (2018) Transcriptome analysis reveals functional roles of nacreous protein N16 in prednisolone-induced osteoporotic zebrafish. International journal of biological macromolecules. 122:1071-1079.
Abstract
N16 is an active protein isolated and screened from nacre proteins. Our previous studies have shown that N16 exerted a good effect on osteoporosis caused by dexamethasone on female rats. In order to further confirm the action of N16 against glucocorticoid-induced osteoporosis (GIOP) and clarify its possible mechanisms, prednisolone-treated larval zebrafish (Danio rerio) model was adopted. Administration of N16 resulted in a significant increase of vertebral bone density of osteoporotic zebrafish, indicating a good effect of N16 against GIOP. Transcriptomic sequencing analysis was then performed to investigate the mechanisms of the action of N16 against GIOP. It was observed that N16 modulated the osteoporotic phenotype by up-regulating the osteoblastic characteristic genes and down-regulating the osteoclastic characteristic genes in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping