PUBLICATION

Association between adaptive immunity and neutrophil dynamics in zebrafish (Danio rerio) infected by a parasitic ciliate

Authors
Jørgensen, L.V.G., Korbut, R., Jeberg, S., Kania, P.W., Buchmann, K.
ID
ZDB-PUB-180913-18
Date
2018
Source
PLoS One   13: e0203297 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Ciliophora Infections/immunology
  • Ciliophora Infections/parasitology
  • Ciliophora Infections/veterinary*
  • Gills/immunology
  • Gills/parasitology
  • Genes, Immunoglobulin
  • Animal Fins/immunology
  • Animal Fins/parasitology
  • Parasite Load
  • Animals
  • Up-Regulation
  • Immunization
  • Fish Diseases/genetics
  • Fish Diseases/immunology*
  • Fish Diseases/parasitology*
  • Hymenostomatida/immunology*
  • Hymenostomatida/pathogenicity*
  • Zebrafish/genetics
  • Zebrafish/immunology*
  • Zebrafish/parasitology*
  • Immunologic Memory/genetics
  • Animals, Genetically Modified
  • Neutrophils/immunology*
  • Host-Parasite Interactions/genetics
  • Host-Parasite Interactions/immunology
  • Adaptive Immunity/genetics
(all 26)
PubMed
30204772 Full text @ PLoS One
Abstract
The protective immune response in zebrafish (Danio rerio) against the parasitic ciliate Ichthyophthirius multifiliis, targeting host skin, fins and gills, comprises an accelerated and manifold elevated immunoglobulin gene expression as well as a significantly elevated number of neutrophils at infected sites. Experimental fish were subjected to a primary I. multifiliis infection followed by a series of secondary exposures before they were challenged by a high dosage of infective theronts. Immunized fish responded immediately with a protective response suggesting existence of immunological memory whereas fish exposed to the parasite for the first time obtained a marked infection. The primary response to infection was dominated by expression of genes encoding acute phase reactants and inflammatory cytokines as well as recruitment of neutrophils at infected locations. Immunized fish showed a significantly upregulated immunoglobulin gene expression following challenge, which indicates existence of a secondary response effected by antibodies. Both responses induced a significantly elevated expression of the Th2 signature cytokine Il13. The increased presence of neutrophils in immunized fish suggests that innate cell mediated immunity supplements or influence the protective response against the parasite.
Genes / Markers
Figures
Figure Gallery (2 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
i114TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    No data available
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    GFPEFGGFP
    1 - 1 of 1
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    Mapping
    No data available
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    Citation
    Jørgensen, L.V.G., Korbut, R., Jeberg, S., Kania, P.W., Buchmann, K. (2018) Association between adaptive immunity and neutrophil dynamics in zebrafish (Danio rerio) infected by a parasitic ciliate. PLoS One. 13:e0203297.