PUBLICATION
Activation of BDNF-TrkB signaling pathway-regulated brain inflammation in pentylenetetrazole-induced seizures in zebrafish
- Authors
- Jin, M., Sheng, W., Han, L., He, Q., Ji, X., Liu, K.
- ID
- ZDB-PUB-180910-3
- Date
- 2018
- Source
- Fish & shellfish immunology 83: 26-36 (Journal)
- Registered Authors
- Keywords
- BDNF, Inflammatory responses, Seizures, TrkB, Zebrafish
- MeSH Terms
-
- Animals
- Brain/drug effects
- Brain-Derived Neurotrophic Factor/genetics*
- Disease Models, Animal
- Encephalitis/chemically induced
- Encephalitis/genetics
- Epilepsy/genetics
- Gene Expression Profiling
- Gene Regulatory Networks
- Membrane Glycoproteins/genetics*
- Pentylenetetrazole
- Receptor, trkB/genetics*
- Seizures/chemically induced
- Seizures/genetics*
- Signal Transduction*
- Zebrafish/physiology*
- PubMed
- 30195910 Full text @ Fish Shellfish Immunol.
Citation
Jin, M., Sheng, W., Han, L., He, Q., Ji, X., Liu, K. (2018) Activation of BDNF-TrkB signaling pathway-regulated brain inflammation in pentylenetetrazole-induced seizures in zebrafish. Fish & shellfish immunology. 83:26-36.
Abstract
Seizures are sustained neuronal hyperexcitability in brain that result in loss of consciousness and injury. Understanding how the brain responds to seizures is critical to help developing new therapeutic strategies for epilepsy, a neurological disorder characterized by recurrent and unprovoked seizures. However, the mechanisms underlying seizure-dependent alterations of biological properties are poorly understood. In this study, we analyzed gene expression profiles of the zebrafish heads that were undergoing seizures and identified 1776 differentially expressed genes. Gene-regulatory network analysis revealed that BDNF-TrkB signaling pathway positively regulated brain inflammation in zebrafish during seizures. Using K252a, a TrkB inhibitor to block BDNF-TrkB signaling pathway, attenuated pentylenetetrazole (PTZ)-induced seizures, which also confirmed BDNF-TrkB mediated inflammatory responses including regulation of il1β and nfκb, and neutrophil and macrophage infiltration of brain. Our results have provided novel insights into seizure-induced brain inflammation in zebrafish and anti-inflammatory related therapy for epilepsy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping