PUBLICATION

Activation of BDNF-TrkB signaling pathway-regulated brain inflammation in pentylenetetrazole-induced seizures in zebrafish

Authors
Jin, M., Sheng, W., Han, L., He, Q., Ji, X., Liu, K.
ID
ZDB-PUB-180910-3
Date
2018
Source
Fish & shellfish immunology   83: 26-36 (Journal)
Registered Authors
Keywords
BDNF, Inflammatory responses, Seizures, TrkB, Zebrafish
MeSH Terms
  • Animals
  • Brain/drug effects
  • Brain-Derived Neurotrophic Factor/genetics*
  • Disease Models, Animal
  • Encephalitis/chemically induced
  • Encephalitis/genetics
  • Epilepsy/genetics
  • Gene Expression Profiling
  • Gene Regulatory Networks
  • Membrane Glycoproteins/genetics*
  • Pentylenetetrazole
  • Receptor, trkB/genetics*
  • Seizures/chemically induced
  • Seizures/genetics*
  • Signal Transduction*
  • Zebrafish/physiology*
PubMed
30195910 Full text @ Fish Shellfish Immunol.
Abstract
Seizures are sustained neuronal hyperexcitability in brain that result in loss of consciousness and injury. Understanding how the brain responds to seizures is critical to help developing new therapeutic strategies for epilepsy, a neurological disorder characterized by recurrent and unprovoked seizures. However, the mechanisms underlying seizure-dependent alterations of biological properties are poorly understood. In this study, we analyzed gene expression profiles of the zebrafish heads that were undergoing seizures and identified 1776 differentially expressed genes. Gene-regulatory network analysis revealed that BDNF-TrkB signaling pathway positively regulated brain inflammation in zebrafish during seizures. Using K252a, a TrkB inhibitor to block BDNF-TrkB signaling pathway, attenuated pentylenetetrazole (PTZ)-induced seizures, which also confirmed BDNF-TrkB mediated inflammatory responses including regulation of il1β and nfκb, and neutrophil and macrophage infiltration of brain. Our results have provided novel insights into seizure-induced brain inflammation in zebrafish and anti-inflammatory related therapy for epilepsy.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping